ArticleProtein science : a publication of the Protein Society2000
The oxidation produced by hydrogen peroxide on Ca-ATP-G-actin.
Article in Protein science : a publication of the Protein Society, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 63 citations in OpenAlex.
- From amino-acid to disease: the effects of oxidation on actin-myosin interactions in muscle.Journal of muscle research and cell motility · 2023Review
- The Potential of a Protein Model Synthesized Absent of Methionine.Molecules (Basel, Switzerland) · 2022Review
- Susceptibility of Protein Methionine Oxidation in Response to Hydrogen Peroxide Treatment-Ex Vivo Versus In Vitro: A Computational Insight.Antioxidants (Basel, Switzerland) · 2020Article
- A single cysteine post-translational oxidation suffices to compromise globular proteins kinetic stability and promote amyloid formation.Redox biology · 2018Article
- Actin filaments-A target for redox regulation.Cytoskeleton (Hoboken, N.J.) · 2016Review
- MICAL-family proteins: Complex regulators of the actin cytoskeleton.Antioxidants & redox signaling · 2014Review
- Reactive oxygen species in inflammation and tissue injury.Antioxidants & redox signaling · 2014Review
- Identification of crucial amino acids of bacterial Peptide deformylases affecting enzymatic activity in response to oxidative stress.Journal of bacteriology · 2014Article
- Comparative proteomic analysis of cysteine oxidation in colorectal cancer patients.Molecules and cells · 2013Article
- The role of transcription-independent damage signals in the initiation of epithelial wound healing.Nature reviews. Molecular cell biology · 2013Review
- Post-translational modification and regulation of actin.Current opinion in cell biology · 2013Review
- Diverse protective roles of the actin cytoskeleton during oxidative stress.Cytoskeleton (Hoboken, N.J.) · 2011Article
- MALDI imaging and profiling MS of higher mass proteins from tissue.Journal of the American Society for Mass Spectrometry · 2010Article
- Features of senescence and cell death induced by doxorubicin in A549 cells: organization and level of selected cytoskeletal proteins.Journal of cancer research and clinical oncology · 2010Article
- Changes in actin structural transitions associated with oxidative inhibition of muscle contraction.Biochemistry · 2008Article
- Functional, structural, and chemical changes in myosin associated with hydrogen peroxide treatment of skeletal muscle fibers.American journal of physiology. Cell physiology · 2008Article
- Skeletal muscle dysfunction in patients with chronic obstructive pulmonary disease.International journal of chronic obstructive pulmonary disease · 2008Review
- Antioxidants preserve macrophage phagocytosis of Pseudomonas aeruginosa during hyperoxia.Free radical biology & medicine · 2007Article
- Structure and activity of the axon guidance protein MICAL.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report here that in vitro exposure of monomeric actin to hydrogen peroxide leads to a conversion of 6 of the 16 methionine residues to methionine sulfoxide residues. Although the initial effect of H2O2 on actin is the oxidation of Cys374, we have found that Met44, Met47, Met176, Met190, Met269, and Met355 are the other sites of the oxidative modification. Met44 and Met47 are the methionyl sites first oxidized. The methionine residues that are oxidized are not simply related to their accessibility to the external medium and are found in all four subdomains of actin. The conformations of subdomain 1, a region critical for the functional binding of different actin-binding proteins, and subdomain 2, which plays important roles in the polymerization process and stabilization of the actin filament, are changed upon oxidation. The conformational changes are deduced from the increased exposure of hydrophobic residues, which correlates with methionine sulfoxide formation, from the perturbations in tryptophan fluorescence, and from the decreased susceptibility to limited proteolysis of oxidized actin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.