ArticleOncogene2000
Aberrant p27Kip1 promoter methylation in malignant melanoma.
Article in Oncogene, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 62 citations in OpenAlex.
- Downregulation of Cyclin Kinase Inhibitors p16INK4a and p27 in Conjunctival Melanomas.Investigative ophthalmology & visual science · 2026Article
- Epigenetic regulation in human melanoma: past and future.Epigenetics · 2015Review
- Quantitative gene analysis of methylation and expression (Q-GAME) in fresh or fixed cells and tissues.Experimental dermatology · 2014Article
- Promoter hypermethylation of p73 and p53 genes in cervical cancer patients among north Indian population.Molecular biology reports · 2012Article
- Article
- Functional modulation of IGF-binding protein-3 expression in melanoma.The Journal of investigative dermatology · 2010Article
- Epigenetics of human cutaneous melanoma: setting the stage for new therapeutic strategies.Journal of translational medicine · 2010Article
- Expression and hypermethylation of p27 kip1 in hepatocarcinogenesis.World journal of gastroenterology · 2005Article
- Promoter methylation profiling of 30 genes in human malignant melanoma.Cancer science · 2004Article
- Loss of heterozygosity at 12p13 and loss of p27KIP1 protein expression contribute to melanoma progression.Virchows Archiv : an international journal of pathology · 2004Article
- Delineation and candidate gene mutation screening of the 18q22 minimal region of deletion in head and neck squamous cell carcinoma.Oncogene · 2002Article
- Significant impact of promoter hypermethylation and the 540 C>T polymorphism of CDKN2A in cutaneous melanoma of the vertical growth phase.The American journal of pathology · 2002Article
- Heterogeneous methylation and deletion patterns of the INK4a/ARF locus within prostate carcinomas.The American journal of pathology · 2002Article
- Methylation matters.Journal of medical genetics · 2001Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
p27Kip1 is a regulator of the mammalian cell cycle and a putative tumor suppressor. Distinct altered patterns of p27Kip1 protein expression are found in a variety of human carcinomas, and p27Kip1 expression levels usually correlate directly with disease-free survival. The mechanism(s) by which p27Kip1 expression is reduced or lost during tumorigenesis remains unclear. Specific alterations of the p27Kip1 gene, including mutations and homozygous deletions, are exceedingly rare in human cancers. We have used methylation-specific PCR and bisulfite genomic sequencing to examine the methylation status of p27Kip1 in 61 primary and metastatic tumors and 35 cell lines from patients with malignant melanoma. Dense methylation of a CpG island in the promoter region of p27Kip1 was detected in four of 45 metastatic tumors (9%) and three of the cell lines (9%), including two cell lines established from two different metastases from the same patient. Examination of a naturally occurring, allele-specific sequence variant demonstrated that p27Kip1 methylation is associated with transcriptional silencing in situ. Cell lines with p27Kip1 methylation showed retention of the wild-type allele and detectable p27Kip1 protein whose abundance was reduced compared with normal melanocytes. Collectively, our data suggest that DNA methylation may be a cause of monoallelic p27Kip1 silencing in malignant melanoma, which would support a role for p27Kip1 haploinsufficiency in human cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.