ArticleThe Biochemical journal2000
Transmembrane and cytoplasmic domains of syndecan mediate a multi-step endocytic pathway involving detergent-insoluble membrane rafts.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The trial behind it
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Who cites it
64 citing papers in PubMed, 146 citations in OpenAlex.
- Gangliosides modulate the secretion of extracellular vesicles and their misfolded protein cargo.Science advances · 2025Article
- Biodistribution and Cellular Internalization of Inactivated SARS-CoV-2 in Wild-Type Mice.International journal of molecular sciences · 2022Article
- Receptor control by membrane-tethered ubiquitin ligases in development and tissue homeostasis.Current topics in developmental biology · 2022Article
- Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus.Developmental cell · 2019Article
- Factors Enhancing Serum Syndecan-1 Concentrations: A Large-Scale Comprehensive Medical Examination.Journal of clinical medicine · 2019Article
- Fibroblast Growth Factor-2 Binding to Heparan Sulfate Proteoglycans Varies with Shear Stress in Flow-Adapted Cells.Annals of biomedical engineering · 2019Article
- The heparan sulfate proteoglycan grip on hyperlipidemia and atherosclerosis.Matrix biology : journal of the International Society for Matrix Biology · 2018Review
- Dually modified transmembrane proteoglycans in development and disease.Cytokine & growth factor reviews · 2018Review
- Heparan Sulfate Proteoglycans as Emerging Players in Synaptic Specificity.Frontiers in molecular neuroscience · 2018Review
- Suppression of Hepatic FLOT1 (Flotillin-1) by Type 2 Diabetes Mellitus Impairs the Disposal of Remnant Lipoproteins via Syndecan-1.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- Heparan sulfate proteoglycans present PCSK9 to the LDL receptor.Nature communications · 2017Article
- Syndecan-1 and Its Expanding List of Contacts.Advances in wound care · 2015Review
- Processing of heparanase is mediated by syndecan-1 cytoplasmic domain and involves syntenin and α-actinin.Cellular and molecular life sciences : CMLS · 2014Article
- SULF2 strongly prediposes to fasting and postprandial triglycerides in patients with obesity and type 2 diabetes mellitus.Obesity (Silver Spring, Md.) · 2014Article
- Reply to "heparan sulfate in baculovirus binding and entry of Mammalian cells".Journal of virology · 2014Article
- The productive entry pathway of HIV-1 in macrophages is dependent on endocytosis through lipid rafts containing CD4.PloS one · 2014Article
- Clathrin-independent endocytosis: a cargo-centric view.Experimental cell research · 2013Review
- 6-o- and N-sulfated syndecan-1 promotes baculovirus binding and entry into Mammalian cells.Journal of virology · 2013Article
- Cell susceptibility to baculovirus transduction and echovirus infection is modified by protein kinase C phosphorylation and vimentin organization.Journal of virology · 2013Article
- Syndecan-4 signaling at a glance.Journal of cell science · 2013Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Syndecan heparan sulphate proteoglycans directly mediate a novel endocytic pathway. Using Chinese hamster ovary cells expressing the human syndecan 1 core protein or a chimaeric receptor, FcR-Synd, consisting of the ectodomain of the IgG Fc receptor Ia linked to the transmembrane and cytoplasmic domains of syndecan 1, we previously reported that efficient internalization is triggered by ligand clustering, requires intact actin microfilaments and tyrosine kinases, proceeds with a t(1/2) of approx. 1 h and is distinct from coated-pit pathways. We have now examined the involvement of cholesterol-rich, detergent-insoluble membrane rafts. On clustering, (125)I-labelled IgG bound to FcR-Synd rapidly became insoluble in cold Triton X-100, well before endocytosis. Insolubility of clustered FcR-Synd ligand did not require the syndecan ectodomain, linkage of the cytoplasmic tail to the cytoskeleton, or energy-dependent cellular metabolism. Pretreatment of cells with cyclodextrin to deplete cholesterol from rafts abolished insolubility of the clustered ligand and inhibited endocytosis in a dose-responsive fashion. Similar results were obtained with (125)I-labelled lipoprotein lipase bound to authentic cell-surface syndecan. In contrast, the 39 kDa receptor-associated protein (RAP), a coated-pit ligand, was more than 80% soluble in cold Triton even after internalization; cellular cholesterol depletion failed to substantially affect the internalization of (125)I-RAP. Overall, our results indicate a multi-step endocytic process consisting of ligand binding, clustering, energy-independent lateral movement into detergent-insoluble membrane rafts and finally recruitment of actin and tyrosine kinases to bring the ligands into the cell.
Indexed as
Identifiers
11042114PMC1221399W1991172636What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.