ArticleProceedings of the National Academy of Sciences of the United States of America1997
Characterization of the transposition pattern of the Ac element in Arabidopsis thaliana using endonuclease I-SceI.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed.
- Efficient generation of marker-free transgenic rice plants using an improved transposon-mediated transgene reintegration strategy.Plant physiology · 2015Article
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- Genome-wide analysis of Tol2 transposon reintegration in zebrafish.BMC genomics · 2009Article
- MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice.Molecular genetics and genomics : MGG · 2008Article
- The WiscDsLox T-DNA collection: an arabidopsis community resource generated by using an improved high-throughput T-DNA sequencing pipeline.Journal of plant research · 2007Article
- Transposable elements in fish functional genomics: technical challenges and perspectives.Genome biology · 2007Review
- Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives.Genome biology · 2007Review
- Article
- The highly conserved family of Tetrahymena thermophila chromosome breakage elements contains an invariant 10-base-pair core.Eukaryotic cell · 2006Article
- Reverse genetic approaches for functional genomics of rice.Plant molecular biology · 2005Review
- Size does matter: cre-mediated somatic deletion efficiency depends on the distance between the target lox-sites.Plant molecular biology · 2005Article
- Site preferences of insertional mutagenesis agents in Arabidopsis.Plant physiology · 2005Article
- An inducible targeted tagging system for localized saturation mutagenesis in Arabidopsis.Plant physiology · 2005Article
- Establishment of an enhancer trap system with Ds and GUS for functional genomics in rice.Molecular genetics and genomics : MGG · 2004Article
- The HY2 gene as an efficient marker for transposon excision in Arabidopsis.Molecular genetics and genomics : MGG · 2003Article
- Resources for targeted insertional and deletional mutagenesis in Arabidopsis.Plant molecular biology · 2003Article
- A resource of mapped dissociation launch pads for targeted insertional mutagenesis in the Arabidopsis genome.Plant physiology · 2003Article
- Continuous exchange of sequence information between dispersed Tc1 transposons in the Caenorhabditis elegans genome.Genetics · 2003Article
- Multi-functional T-DNA/Ds tomato lines designed for gene cloning and molecular and physical dissection of the tomato genome.Plant molecular biology · 2003Article
- Rice genome organization: the centromere and genome interactions.Annals of botany · 2002Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated physical distances and directions of transposition of the maize transposable element Ac in Arabidopsis thaliana. We prepared a transferred DNA (T-DNA) construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-SceI (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. Three transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. These transgenic plants were crossed with the Arabidopsis that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with endonuclease I-SceI, sizes of segment of DNA were determined by pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results showed that 50% of all transposition events had occurred within 1,700 kb on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites.
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