ArticleProceedings of the National Academy of Sciences of the United States of America1997
Purified vesicles of tobacco cell vacuolar and plasma membranes exhibit dramatically different water permeability and water channel activity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 183 citations in OpenAlex.
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- Genome-Wide Identification of Maize Aquaporin and Functional Analysis During Seed Germination and Seedling Establishment.Frontiers in plant science · 2022Article
- Genome-Wide Identification, Structure Characterization, and Expression Pattern Profiling of the Aquaporin Gene Family inInternational journal of molecular sciences · 2021Article
- Age dependence of the hydraulic resistances of the plasma membrane and the tonoplast (vacuolar membrane) in cells of Chara corallina.Protoplasma · 2021Article
- Plasma membrane vesicles from cauliflower meristematic tissue and their role in water passage.BMC plant biology · 2021Article
- Genome-wide analysis of the aquaporin genes in melon (Cucumis melo L.).Scientific reports · 2020Article
- Nanobiofertilization as a novel technology for highly efficient foliar application of Fe and B in almond trees.Royal Society open science · 2020Article
- Detergent Resistant Membrane Domains in Broccoli Plasma Membrane Associated to the Response to Salinity Stress.International journal of molecular sciences · 2020Article
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- Tension-activated channels in the mechanism of osmotic fitness inThe Journal of general physiology · 2017Article
- Tonoplast Aquaporins Facilitate Lateral Root Emergence.Plant physiology · 2016Article
- The Roles of Aquaporins in Plant Stress Responses.Journal of developmental biology · 2016Review
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- Genome-wide comparative analysis of tonoplast intrinsic protein (TIP) genes in plants.Functional & integrative genomics · 2014Review
- Expression of tomato SlTIP2;2 enhances the tolerance to salt stress in the transgenic Arabidopsis and interacts with target proteins.Journal of plant research · 2014Article
- Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit.Journal of nucleic acids · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The vacuolar membrane or tonoplast (TP) and the plasma membrane (PM) of tobacco suspension cells were purified by free-flow electrophoresis (FFE) and aqueous two-phase partitioning, with enrichment factors from a crude microsomal fraction of >/=4- to 5-fold and reduced contamination by other cellular membranes. For each purified fraction, the mean apparent diameter of membrane vesicles was determined by freeze-fracture electron microscopy, and the osmotic shrinking kinetics of the vesicles were characterized by stopped-flow light scattering. Osmotic water permeability coefficients (Pf) of 6.1 +/- 0.2 and 7.6 +/- 0.9 microm . s(-1) were deduced for PM-enriched vesicles purified by FFE and phase partitioning, respectively. The associated activation energies (Ea; 13.7 +/- 1.0 and 13.4 +/- 1.4 kcal . mol(-1), respectively) suggest that water transport in the purified PM occurs mostly by diffusion across the lipid matrix. In contrast, water transport in TP vesicles purified by FFE was characterized by (i) a 100-fold higher Pf of 690 +/- 35 microm . s(-1), (ii) a reduced Ea of 2.5 +/- 1.3 kcal . mol(-1), and (iii) a reversible inhibition by mercuric chloride, up to 83% at 1 mM. These results provide functional evidence for channel-mediated water transport in the TP, and more generally in a higher plant membrane. A high TP Pf suggests a role for the vacuole in buffering osmotic fluctuations occurring in the cytoplasm. Thus, the differential water permeabilities and water channel activities observed in the tobacco TP and PM point to an original osmoregulatory function for water channels in relation to the typical compartmentation of plant cells.
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