ArticleProceedings of the National Academy of Sciences of the United States of America1997
RecA-like proteins are components of early meiotic nodules in lily.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 148 citations in OpenAlex.
- Disparate roles for C. elegans DNA translocase paralogs RAD-54.L and RAD-54.B in meiotic prophase germ cells.Nucleic acids research · 2023Article
- Crossover recombination and synapsis are linked by adjacent regions within the N terminus of the Zip1 synaptonemal complex protein.PLoS genetics · 2019Article
- Immunolocalization of chromosome-associated proteins in plants - principles and applications.Botanical studies · 2014Review
- Mechanism and regulation of meiotic recombination initiation.Cold Spring Harbor perspectives in biology · 2014Review
- Licensing MLH1 sites for crossover during meiosis.Nature communications · 2014Article
- On the role of AtDMC1, AtRAD51 and its paralogs during Arabidopsis meiosis.Frontiers in plant science · 2014Review
- Altered distribution of MLH1 foci is associated with changes in cohesins and chromosome axis compaction in an asynaptic mutant of tomato.Chromosoma · 2012Article
- RAD51-associated protein 1 (RAD51AP1) interacts with the meiotic recombinase DMC1 through a conserved motif.The Journal of biological chemistry · 2011Article
- Genetic interference: don't stand so close to me.Current genomics · 2010Article
- A high throughput genetic screen identifies new early meiotic recombination functions in Arabidopsis thaliana.PLoS genetics · 2009Article
- Chromatin remodeling finds its place in the DNA double-strand break response.Nucleic acids research · 2009Review
- Cytological analysis of MRE11 protein during early meiotic prophase I in Arabidopsis and tomato.Chromosoma · 2008Article
- Dissecting meiosis of rye using translational proteomics.Annals of botany · 2008Article
- Article
- Zip4/Spo22 is required for class I CO formation but not for synapsis completion in Arabidopsis thaliana.PLoS genetics · 2007Article
- The mismatch repair protein MLH1 marks a subset of strongly interfering crossovers in tomato.The Plant cell · 2007Article
- The cellular control of DNA double-strand breaks.Journal of cellular biochemistry · 2006Review
- Two levels of interference in mouse meiotic recombination.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Article
- A molecular portrait of Arabidopsis meiosis.The arabidopsis book · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early meiotic nodules (also called recombination nodules) are proteinaceous structures about 100 nm in diameter that are associated with forming synaptonemal complexes (SCs) during early prophase I of meiosis. Although their function is unknown, early nodules may be involved in searching for DNA homology before synaptic initiation. Two potential components of early nodules are Rad51 and Dmc1 proteins. These proteins are important for meiotic recombination in eukaryotes and are homologous to RecA, the major protein that catalyzes homologous pairing and DNA strand exchange in prokaryotes. In addition, Rad51 has been localized by immunofluorescence in abundant foci that may correspond to early nodules in yeast, lily, and mouse. In yeast and lily, Dmc1 and Lim15, the lily homolog of Dmc1, colocalize with Rad51. Here, using electron microscopic immunogold localization to spreads of zygotene and early pachytene SCs from lily, we confirm that RecA-like proteins are components of early nodules. The antibody used was generated to full-length tomato Rad51 protein and binds to both Rad51 and Lim15 in immunoblots of lily primary microsporocyte proteins. The labeled early nodules are heterogeneous in size and are associated with both axial elements and SCs. There are two classes of early nodules, those that are densely labeled with gold and those that are not labeled at all. This result may be due to technical limitations associated with using spread preparations or to differences in the nodules themselves. The presence of Rad51 and/or Lim15 proteins in early nodules supports the hypothesis that early nodules are involved in recombination-related events during meiosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.