ArticleProceedings of the National Academy of Sciences of the United States of America1997
Ca2+-independent and Ca2+/GTP-binding protein-controlled exocytosis in a plant cell.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 57 citations in OpenAlex.
- Could vesicular transport of Na+ and Cl- be a feature of salt tolerance in halophytes?Annals of botany · 2019Article
- Calcium: The Missing Link in Auxin Action.Plants (Basel, Switzerland) · 2013Review
- Exocytosis precedes and predicts the increase in growth in oscillating pollen tubes.The Plant cell · 2009Article
- A distinct endosomal Ca2+/Mn2+ pump affects root growth through the secretory process.Plant physiology · 2008Article
- Secretion marker proteins and cell-wall polysaccharides move through different secretory pathways.Planta · 2007Article
- Correlative Analysis of [Ca](C) and Apical Secretion during Pollen Tube Growth and Reorientation.Plant signaling & behavior · 2006Article
- Selective mobility and sensitivity to SNAREs is exhibited by the Arabidopsis KAT1 K+ channel at the plasma membrane.The Plant cell · 2006Article
- Sorting of GFP Tagged NtSyr1, an ABA Related Syntaxin.Plant signaling & behavior · 2006Article
- Modulation of endocytosis in pollen tube growth by phosphoinositides and phospholipids.Protoplasma · 2005Review
- The regulation of anion loading to the maize root xylem.Plant physiology · 2005Article
- The abscisic acid-related SNARE homolog NtSyr1 contributes to secretion and growth: evidence from competition with its cytosolic domain.The Plant cell · 2002Article
- Heterotrimeric and unconventional GTP binding proteins in plant cell signaling.The Plant cell · 2002Review
- Abscisic acid stimulation of phospholipase D in the barley aleurone is G-protein-mediated and localized to the plasma membrane.Plant physiology · 2000Article
- Ca(2)+-stimulated exocytosis in maize coleoptile cells.The Plant cell · 2000Article
- Exocytosis and endocytosisThe Plant cell · 1999Article
- Exocytosis in plants.Plant molecular biology · 1998Review
- Ca2+, annexins, and GTP modulate exocytosis from maize root cap protoplastsThe Plant cell · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exocytosis allows the release of secretory products and the delivery of new membrane material to the plasma membrane. So far, little is known about the underlying molecular mechanism and its control in plant cells. We have used the whole-cell patch-clamp technique to monitor changes in membrane capacitance to study exocytosis in barley aleurone protoplasts. To investigate the involvement of Ca2+ and GTP-binding proteins in exocytosis, protoplasts were dialyzed with very low (<2 nM) and high (1 microM) free Ca2+ and nonhydrolyzable guanine nucleotides guanosine 5'-gamma-thio]triphosphate (GTP[gammaS]) or guanosine 5'-[beta-thio]diphosphate (GDP[betaS]). With less than 2 nM cytoplasmic free Ca2+, the membrane capacitance increased significantly over 20 min. This increase was not altered by GTP[gammaS] or GDP[betaS]. In contrast, dialyzing protoplasts with 1 microM free Ca2+ resulted in a large increase in membrane capacitance that was slightly reduced by GTP[gammaS] and strongly inhibited by GDP[betaS]. We conclude that two exocytotic pathways exist in barley aleurone protoplasts: one that is Ca2+-independent and whose regulation is currently not known and another that is stimulated by Ca2+ and modulated by GTP-binding proteins. We suggest that Ca2+-independent exocytosis may be involved in cell expansion in developing protoplasts. Ca2+-stimulated exocytosis may play a role in gibberellic acid-stimulated alpha-amylase secretion in barley aleurone and, more generally, may be involved in membrane resealing in response to cell damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.