ArticleThe Journal of cell biology2000
Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
88 citing papers in PubMed, 194 citations in OpenAlex.
- Alleviation of ER stress via targeted genetic engineering enhances recombinant ovalbumin secretion inFood science and biotechnology · 2026Article
- Endocytosis and trans-Golgi Network in Yeast.Sub-cellular biochemistry · 2026Review
- P4-ATPase endosomal recycling relies on multiple retromer-dependent localization signals.Molecular biology of the cell · 2024Article
- Role of phosphatidylserine in the localization of cell surface membrane proteins in yeast.Cell structure and function · 2023Article
- Retromer subunit, CfVps35 is required for growth development and pathogenicity of Colletotrichum fructicola.BMC genomic data · 2022Article
- Retrograde transport of CDMPR depends on several machineries as analyzed by sulfatable nanobodies.Life science alliance · 2022Article
- The Combination of Iron and Copper Increases Pathogenicity and Induces Proteins Related to the Main Virulence Factors in Clinical Isolates ofJournal of fungi (Basel, Switzerland) · 2022Article
- Clathrin adaptors mediate two sequential pathways of intra-Golgi recycling.The Journal of cell biology · 2022Article
- Article
- Analysis of the SNARE Stx8 recycling reveals that the retromer-sorting motif has undergone evolutionary divergence.PLoS genetics · 2021Article
- Navigating the Controversies of Retromer-Mediated Endosomal Protein Sorting.Frontiers in cell and developmental biology · 2021Review
- Targeting of Lysosomal Pathway Genes for Parkinson's Disease Modification: Insights From Cellular and Animal Models.Frontiers in neurology · 2021Review
- The ESCRT machinery regulates retromer-dependent transcytosis of septate junction components ineLife · 2020Article
- Endosome-to-TGN Trafficking: Organelle-Vesicle and Organelle-Organelle Interactions.Frontiers in cell and developmental biology · 2020Review
- A bipartite sorting signal ensures specificity of retromer complex in membrane protein recycling.The Journal of cell biology · 2019Article
- Retromer and the cation-independent mannose 6-phosphate receptor-Time for a trial separation?Traffic (Copenhagen, Denmark) · 2018Review
- Autophagic clearance of proteasomes in yeast requires the conserved sorting nexin Snx4.The Journal of biological chemistry · 2017Article
- Updated Insight into the Physiological and Pathological Roles of the Retromer Complex.International journal of molecular sciences · 2017Review
- Systems-wide Studies Uncover Commander, a Multiprotein Complex Essential to Human Development.Cell systems · 2017Review
- A CDC25 family protein phosphatase gates cargo recognition by the Vps26 retromer subunit.eLife · 2017Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Resident late-Golgi membrane proteins in Saccharomyces cerevisiae are selectively retrieved from a prevacuolar-endosomal compartment, a process dependent on aromatic amino acid-based sorting determinants on their cytosolic domains. The formation of retrograde vesicles from the prevacuolar compartment and the selective recruitment of vesicular cargo are thought to be mediated by a peripheral membrane retromer protein complex. We previously described mutations in one of the retromer subunit proteins, Vps35p, which caused cargo-specific defects in retrieval. By genetic and biochemical means we now show that Vps35p directly associates with the cytosolic domains of cargo proteins. Chemical cross-linking, followed by coimmunoprecipitation, demonstrated that Vps35p interacts with the cytosolic domain of A-ALP, a model late-Golgi membrane protein, in a retrieval signal-dependent manner. Furthermore, mutations in the cytosolic domains of A-ALP and another cargo protein, Vps10p, were identified that suppressed cargo-specific mutations in Vps35p but did not suppress the retrieval defects of a vps35 null mutation. Suppression was shown to be due to an improvement in protein sorting at the prevacuolar compartment. These data strongly support a model in which Vps35p acts as a "receptor" protein for recognition of the retrieval signal domains of cargo proteins during their recruitment into retrograde vesicles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.