Evidence map›Paper›PMID 11036646›Full record

ArticleMethods in enzymology2000

Purification of proteins using polyhistidine affinity tags.

J A Bornhorst, J J Falke

Abstract readComparative Study
In one paragraph

Article in Methods in enzymology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 191 papers.

0numbers the graph read from it
0cells of the map it votes in
191citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

191 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Other Techniques.Advances in biochemical engineering/biotechnology · 2026
    Review
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Cuspidatyl Ferulate, a Novel Phenolic Acid fromAntioxidants (Basel, Switzerland) · 2025
    Article

131 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

J A BornhorstDepartment of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215, USA.
J J Falke

Funding

RECEPTORS &SIGNALING PROTEINS--DYNAMICS &ACTIVATIONR01GM040731 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI FALKE, JOSEPH J · 1988 to 2013
$3.4M
MOLECULAR STUDIES OF CALCIUM BINDING AND SIGNALINGR01GM048203 · NIGMS · UNIVERSITY OF COLORADO AT BOULDER · PI FALKE, JOSEPH J · 1992 to 2000
$146k
NIGMS NIH HHS GM 40731NIGMS NIH HHS GM 48203NIGMS NIH HHS R01 GM040731
6 · The paper itself

Abstract

PubMed holds no abstract for this paper.

Indexed as

PeptidesChromatography, AffinityElectrophoresis, Polyacrylamide GelHistidineIndicators and ReagentsMembrane ProteinsNickelNitrilotriacetic AcidOrganometallic CompoundsRecombinant ProteinsHistidineIndicators and ReagentsMembrane ProteinsNickelnickel nitrilotriacetic acidNitrilotriacetic AcidOrganometallic CompoundsPeptidespolyhistidineRecombinant Proteins

Identifiers

PMID11036646
PMCPMC2909483

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.