ArticleAntimicrobial agents and chemotherapy2000
Simultaneous quantitation of intracellular zidovudine and lamivudine triphosphates in human immunodeficiency virus-infected individuals.
Article in Antimicrobial agents and chemotherapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 59 citations in OpenAlex.
- Intracellular pharmacokinetics of once versus twice daily zidovudine and lamivudine in adolescents.Antimicrobial agents and chemotherapy · 2007Trial
- Effect of ribavirin on intracellular and plasma pharmacokinetics of nucleoside reverse transcriptase inhibitors in patients with human immunodeficiency virus-hepatitis C virus coinfection: results of a randomized clinical study.Antimicrobial agents and chemotherapy · 2005Trial
- Review
- Direct and indirect quantification of phosphate metabolites of nucleoside analogs in biological samples.Journal of pharmaceutical and biomedical analysis · 2020Review
- A Pharmacokinetic/Pharmacodynamic Model to Predict Effective HIV Prophylaxis Dosing Strategies for People Who Inject Drugs.The Journal of pharmacology and experimental therapeutics · 2018Article
- Simultaneous quantification of intracellular lamivudine and abacavir triphosphate metabolites by LC-MS/MS.Journal of pharmaceutical and biomedical analysis · 2018Article
- Pharmacokinetic Modeling of Lamivudine and Zidovudine Triphosphates Predicts Differential Pharmacokinetics in Seminal Mononuclear Cells and Peripheral Blood Mononuclear Cells.Antimicrobial agents and chemotherapy · 2015Article
- Effect of HIV-1 infection and sex on the cellular pharmacology of the antiretroviral drugs zidovudine and lamivudine.Antimicrobial agents and chemotherapy · 2012Article
- Determination of nucleoside analog mono-, di-, and tri-phosphates in cellular matrix by solid phase extraction and ultra-sensitive LC-MS/MS detection.Journal of pharmaceutical and biomedical analysis · 2011Article
- Intracellular Pharmacokinetics of Antiretroviral Drugs in HIV-Infected Patients, and their Correlation with Drug Action.Clinical pharmacokinetics · 2010Review
- Zidovudine and Lamivudine for HIV Infection.Clinical medicine reviews in therapeutics · 2010Article
- HPLC-MS/MS method for the intracellular determination of ribavirin monophosphate and ribavirin triphosphate in CEM ss cells.Journal of pharmaceutical and biomedical analysis · 2009Article
- Development of an optimized dose for coformulation of zidovudine with drugs that select for the K65R mutation using a population pharmacokinetic and enzyme kinetic simulation model.Antimicrobial agents and chemotherapy · 2008Article
- Differential extracellular and intracellular concentrations of zidovudine and lamivudine in semen and plasma of HIV-1-infected men.Journal of acquired immune deficiency syndromes (1999) · 2008Article
- Is stavudine triphosphate a natural metabolite of zidovudine?Antimicrobial agents and chemotherapy · 2006Article
- Lack of evidence for in vivo transformation of zidovudine triphosphate to stavudine triphosphate in human immunodeficiency virus-infected patients.Antimicrobial agents and chemotherapy · 2006Article
- K65R-associated virologic failure in HIV-infected patients receiving tenofovir-containing triple nucleoside/nucleotide reverse transcriptase inhibitor regimens.MedGenMed : Medscape general medicine · 2004Review
- Determination of ddATP levels in human immunodeficiency virus-infected patients treated with dideoxyinosine.Antimicrobial agents and chemotherapy · 2004Article
- Lamivudine can exert a modest antiviral effect against human immunodeficiency virus type 1 containing the M184V mutation.Antimicrobial agents and chemotherapy · 2003Article
- Enzymatic assay for measurement of intracellular DXG triphosphate concentrations in peripheral blood mononuclear cells from human immunodeficiency virus type 1-infected patients.Antimicrobial agents and chemotherapy · 2003Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Highly active antiretroviral therapy (HAART) is the standard treatment for infection with human immunodeficiency virus (HIV). The most common HAART regimen consists of the combination of at least one protease inhibitor (PI) with two nucleoside reverse transcriptase inhibitors (NRTIs). Contrary to PIs, NRTIs require intracellular activation from the parent compound of their triphosphate moiety to suppress HIV replication. Simultaneous intracellular determination of two NRTI triphosphates is difficult to accomplish due to their relatively small concentrations in peripheral blood mononuclear cells (PBMCs), requiring large amounts of blood from HIV-positive patients. Recently, we described a method to determine intracellular zidovudine triphosphate (ZDV-TP) concentrations in HIV-infected patients by using solid-phase extraction and tandem mass spectrometry. The limit of quantitation (LOQ) for ZDV-TP was 0.10 pmol, and the method was successfully used for the determination of ZDV-TP in HIV-positive patients. In this study, we enhanced the aforementioned method by the simultaneous quantitation of ZDV-TP and lamivudine triphosphate (3TC-TP) in PBMCs from HIV-infected patients. The LOQ for 3TC-TP was 4.0 pmol, with an interassay coefficient of variation and an accuracy of 7 and 12%, respectively. This method was successfully applied to the simultaneous in vivo determination of the ZDV-TP and 3TC-TP pharmacokinetic profiles from HIV-infected patients receiving HAART.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.