ArticleAntimicrobial agents and chemotherapy2000
Genetic analysis of azole resistance in the Darlington strain of Candida albicans.
Article in Antimicrobial agents and chemotherapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 75 citations in OpenAlex.
- Machine learning identifies novel signatures of antifungal drug resistance in Saccharomycotina yeasts.PLoS genetics · 2026Article
- Molecular Identification of Fungal Species through Multiplex-qPCR to Determine Candidal Vulvovaginitis and Antifungal Susceptibility.Journal of fungi (Basel, Switzerland) · 2023Article
- Emergence of Antifungal Resistant Subclades in the Global Predominant Phylogenetic Population of Candida albicans.Microbiology spectrum · 2023Article
- Confirmation of fifthEmerging microbes & infections · 2022Article
- Structural Insights into the Azole Resistance of theJournal of fungi (Basel, Switzerland) · 2021Article
- Detection of ERG11 point mutations in Iranian fluconazole-resistantCurrent medical mycology · 2019Article
- LimitedAntimicrobial agents and chemotherapy · 2018Article
- Fluconazole resistance inInfection and drug resistance · 2017Review
- Triazole resistance mediated by mutations of a conserved active site tyrosine in fungal lanosterol 14α-demethylase.Scientific reports · 2016Article
- The development of fluconazole resistance in Candida albicans - an example of microevolution of a fungal pathogen.Journal of microbiology (Seoul, Korea) · 2016Review
- Structural and Functional Elucidation of Yeast Lanosterol 14α-Demethylase in Complex with Agrochemical Antifungals.PloS one · 2016Article
- A combination approach to treating fungal infections.Scientific reports · 2015Article
- Amphiphilic Tobramycin Analogues as Antibacterial and Antifungal Agents.Antimicrobial agents and chemotherapy · 2015Article
- Contribution of clinically derived mutations in ERG11 to azole resistance in Candida albicans.Antimicrobial agents and chemotherapy · 2015Article
- Structural basis for heterogeneous phenotype of ERG11 dependent Azole resistance in C.albicans clinical isolates.SpringerPlus · 2014Article
- The [URE3] prion in Candida.Eukaryotic cell · 2013Article
- S279 point mutations in Candida albicans Sterol 14-α demethylase (CYP51) reduce in vitro inhibition by fluconazole.Antimicrobial agents and chemotherapy · 2012Article
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- Azole binding properties of Candida albicans sterol 14-alpha demethylase (CaCYP51).Antimicrobial agents and chemotherapy · 2010Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
High-level azole resistance in the Darlington strain of Candida albicans was investigated by gene replacement in C. albicans and expression in Saccharomyces cerevisiae. We sequenced the ERG11 gene, which encodes the sterol C(14)alpha-demethylase, from our copy of the Darlington strain. Both alleles contained the histidine for tyrosine substitution at position 132 (Y132H) reported in Darlington by others, but we also found a threonine-for-isoleucine substitution (I471T) not previously reported in the C. albicans ERG11. The encoded I471T change in amino acids conferred azole resistance when overexpressed alone and increased azole resistance when added to the Y132H amino acid sequence in an S. cerevisiae expression system. Replacement of one copy of ERG11 in an azole-susceptible strain of C. albicans with a single copy of the Darlington ERG11 resulted in expression of the integrated copy and a modest increase in azole resistance. The profound azole resistance of the Darlington strain is the result of multiple mutations.
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