ArticleInfection and immunity2000
Construction of a Vibrio cholerae vaccine candidate using transposon delivery and FLP recombinase-mediated excision.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- DNA Vaccines in the Post-mRNA Era: Engineering, Applications, and Emerging Innovations.International journal of molecular sciences · 2025Review
- Serotype conversion geneFrontiers in microbiology · 2023Article
- The Type II Secretory System Mediates Phage Infection inFrontiers in cellular and infection microbiology · 2021Article
- The outer-membrane protein TolC ofThe Journal of biological chemistry · 2018Article
- Application of FLP-FRT System to Construct Unmarked Deletion inFrontiers in microbiology · 2017Article
- FLP-FRT-based method to obtain unmarked deletions of CHU_3237 (porU) and large genomic fragments of Cytophaga hutchinsonii.Applied and environmental microbiology · 2014Article
- Novel gene clusters and metabolic pathway involved in 3,5,6-trichloro-2-pyridinol degradation by Ralstonia sp. strain T6.Applied and environmental microbiology · 2013Article
- Sequence polymorphisms of rfbT among the Vibrio cholerae O1 strains in the Ogawa and Inaba serotype shifts.BMC microbiology · 2013Article
- O antigen is the receptor of Vibrio cholerae serogroup O1 El Tor typing phage VP4.Journal of bacteriology · 2013Article
- Transcutaneous immunization with a Vibrio cholerae O1 Ogawa synthetic hexasaccharide conjugate following oral whole-cell cholera vaccination boosts vibriocidal responses and induces protective immunity in mice.Clinical and vaccine immunology : CVI · 2012Article
- An experimental study of phage mediated bactericidal selection & emergence of the El Tor Vibrio cholerae.The Indian journal of medical research · 2011Article
- Genetic analysis of activation of the Vibrio cholerae Cpx pathway.Journal of bacteriology · 2009Article
- Article
- 2,3-butanediol synthesis and the emergence of the Vibrio cholerae El Tor biotype.Infection and immunity · 2006Article
- Vibrio cholerae virulence regulator-coordinated evasion of host immunity.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Comparative study of class 1 integron and Vibrio cholerae superintegron integrase activities.Journal of bacteriology · 2005Article
- Establishment of a genetically marked insect-derived symbiont in multiple host plants.Current microbiology · 2005Article
- Unexpected stability of mariner transgenes in Drosophila.Genetics · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Recent efforts to develop a vaccine against the diarrheal disease cholera have focused on the use of live attenuated strains of the causative organism, Vibrio cholerae. The Ogawa lipopolysaccharide phenotype is expressed by many epidemic strains, and motility defects reduce the risk of reactive diarrhea in vaccine recipients. We therefore converted a motile Inaba(+) vaccine candidate, Peru-2, to a nonmotile Ogawa(+) phenotype using a mariner-based transposon carrying rfbT, the gene required for expression of the Ogawa phenotype. Analysis of 22 nonmotile Peru-2 mutants showed that two were Ogawa(+), and both of these strains had insertions in the flgE gene. It was possible to convert these strains to antibiotic sensitivity by introducing a recombinase that acts on sites flanking the antibiotic marker on the transposon. The resulting strains are competent for colonization in infant mice and may therefore be suitable as vaccine candidates for use either independently or in a combination with strains of different biotypes and serotypes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.