ArticleThe EMBO journal2000
Signal transduction between a membrane-bound transporter, PtsG, and a soluble transcription factor, Mlc, of Escherichia coli.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 139 citations in OpenAlex.
- Developing hybrid thiomaltodextrins as specific ligands to the maltodextrin transporter ofRSC advances · 2026Article
- CRP-dependent DNA anchoring and membrane sequestration define a dual mechanism for MtlR-mediated regulation.Nucleic acids research · 2026Article
- Structural basis of Mlc-mediated transcriptional regulation of carbohydrate metabolism.Nature communications · 2026Article
- Control of flagellar gene expression by a chemotaxis receptor-like regulator in pathogenic Escherichia coli.The EMBO journal · 2025Article
- Flipping the switch: dynamic modulation of membrane transporter activity in bacteria.Microbiology (Reading, England) · 2023Review
- How Bacterial Pathogens Coordinate Appetite with Virulence.Microbiology and molecular biology reviews : MMBR · 2023Review
- Sequestration of a dual function DNA-binding protein byProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Sensory Systems and Transcriptional Regulation inFrontiers in bioengineering and biotechnology · 2022Review
- Tyrosine phosphorylation-dependent localization of TmaR that controls activity of a major bacterial sugar regulator by polar sequestration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- High-throughput mapping of the phage resistance landscape in E. coli.PLoS biology · 2020Article
- Transporters of glucose and other carbohydrates in bacteria.Pflugers Archiv : European journal of physiology · 2020Review
- Stochastic Analysis Demonstrates the Dual Role of Hfq in ChaperoningFrontiers in molecular biosciences · 2020Article
- Parallel experimental evolution reveals a novel repressive control of GalP on xylose fermentation in Escherichia coli.Biotechnology and bioengineering · 2019Article
- HilE Regulates HilD by Blocking DNA Binding in Salmonella enterica Serovar Typhimurium.Journal of bacteriology · 2018Article
- The general PTS component HPr determines the preference for glucose over mannitol.Scientific reports · 2017Article
- Regulation of CsrB/C sRNA decay by EIIA(Glc) of the phosphoenolpyruvate: carbohydrate phosphotransferase system.Molecular microbiology · 2016Article
- Dephosphorylated NPr is involved in an envelope stress response of Escherichia coli.Microbiology (Reading, England) · 2015Article
- The transcription factor Mlc promotes Vibrio cholerae biofilm formation through repression of phosphotransferase system components.Journal of bacteriology · 2014Article
- Ex uno plures: clonal reinforcement drives evolution of a simple microbial community.PLoS genetics · 2014Article
- The bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system: regulation by protein phosphorylation and phosphorylation-dependent protein-protein interactions.Microbiology and molecular biology reviews : MMBR · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The global regulator Mlc controls several genes implicated in sugar utilization systems, notably the phosphotransferase system (PTS) genes, ptsG, manXYZ and ptsHI, as well as the malT activator. No specific low molecular weight inducer has been identified that can inactivate Mlc, but its activity appeared to be modulated by transport of glucose via Enzyme IICB(Glc) (PtsG). Here we demonstrate that inactivation of Mlc is achieved by sequestration of Mlc to membranes containing dephosphorylated Enzyme IICB(Glc). We show that Mlc binds specifically to membrane fractions which carry PtsG and that excess Mlc can inhibit Enzyme IICB(Glc) phosphorylation by the general PTS proteins and also Enzyme IICB(Glc)-mediated phosphorylation of alpha-methylglucoside. Binding of Mlc to Enzyme IICB(Glc) in vitro required the IIB domain and the IIC-B junction region. Moreover, we show that these same regions are sufficient for Mlc regulation in vivo, via cross-dephosphorylation of IIB(Glc) during transport of other PTS sugars. The control of Mlc activity by sequestration to a transport protein represents a novel form of signal transduction in gene regulation.
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