ArticleThe EMBO journal2000
A novel regulatory role of glucose transporter of Escherichia coli: membrane sequestration of a global repressor Mlc.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Developing hybrid thiomaltodextrins as specific ligands to the maltodextrin transporter ofRSC advances · 2026Article
- CRP-dependent DNA anchoring and membrane sequestration define a dual mechanism for MtlR-mediated regulation.Nucleic acids research · 2026Article
- Structural basis of Mlc-mediated transcriptional regulation of carbohydrate metabolism.Nature communications · 2026Article
- Unveiling the novel regulatory roles of RpoD-family sigma factors in Salmonella Typhimurium heat shock response through systems biology approaches.PLoS genetics · 2024Article
- How Bacterial Pathogens Coordinate Appetite with Virulence.Microbiology and molecular biology reviews : MMBR · 2023Review
- Tyrosine phosphorylation-dependent localization of TmaR that controls activity of a major bacterial sugar regulator by polar sequestration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Parallel experimental evolution reveals a novel repressive control of GalP on xylose fermentation in Escherichia coli.Biotechnology and bioengineering · 2019Article
- HilE Regulates HilD by Blocking DNA Binding in Salmonella enterica Serovar Typhimurium.Journal of bacteriology · 2018Article
- Trigger phosphodiesterases as a novel class of c-di-GMP effector proteins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Regulation of CsrB/C sRNA decay by EIIA(Glc) of the phosphoenolpyruvate: carbohydrate phosphotransferase system.Molecular microbiology · 2016Article
- Dephosphorylated NPr is involved in an envelope stress response of Escherichia coli.Microbiology (Reading, England) · 2015Article
- The transcription factor Mlc promotes Vibrio cholerae biofilm formation through repression of phosphotransferase system components.Journal of bacteriology · 2014Article
- The bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system: regulation by protein phosphorylation and phosphorylation-dependent protein-protein interactions.Microbiology and molecular biology reviews : MMBR · 2014Review
- HPr antagonizes the anti-σ70 activity of Rsd in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Structure-function studies of the staphylococcal methicillin resistance antirepressor MecR2.The Journal of biological chemistry · 2013Article
- The EAL domain protein YciR acts as a trigger enzyme in a c-di-GMP signalling cascade in E. coli biofilm control.The EMBO journal · 2013Article
- Reciprocal regulation of the autophosphorylation of enzyme INtr by glutamine and α-ketoglutarate in Escherichia coli.Molecular microbiology · 2013Article
- Borrelia host adaptation Regulator (BadR) regulates rpoS to modulate host adaptation and virulence factors in Borrelia burgdorferi.Molecular microbiology · 2013Article
- Metabolic Regulation of a Bacterial Cell System with Emphasis on Escherichia coli Metabolism.ISRN biochemistry · 2013Review
- Glucose transport in Escherichia coli mutant strains with defects in sugar transport systems.Journal of bacteriology · 2012Article
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Authors and funding
3 authors.
Funding
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Abstract
External glucose stimulates transcription of several genes including ptsG encoding IICB(Glc), a membrane component of the phosphotransferase system (PTS), by relieving the negative regulation of a global repressor Mlc in Escherichia coli. We investigate here how glucose modulates Mlc action. The Mlc-mediated repression is eliminated by a ptsI mutation, while Mlc is constitutively active in a ptsG mutant. We show that IICB(Glc)-FLAG interacts physically with Mlc in crude extracts prepared from cells in which IICB(Glc) is supposed to exist as the non-phosphorylated form. The IICB(Glc)-Mlc interaction is no longer observed when IICB(Glc) is phosphorylated. Exogenously added purified Mlc binds to purified IICB(Glc)-FLAG. We also demonstrate that Mlc is associated with membrane when IICB(Glc) is dephosphorylated while it is in the cytoplasm when IICB(Glc) is phosphorylated or absent. We conclude that IICB(Glc) regulates the cellular localization of Mlc, depending on its phosphorylation state, which is determined by the availability of external glucose. Thus, glucose induces the transcription of Mlc-regulated promoters by sequestering Mlc to the membrane through dephosphorylation of IICB(Glc).
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