ArticleTransgenic research2000
Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat.
Article in Transgenic research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 50 citations in OpenAlex.
- Use of Genome Editing Techniques to Produce Transgenic Farm Animals.Advances in experimental medicine and biology · 2022Review
- Transcriptome profile of goat folliculogenesis reveals the interaction of oocyte and granulosa cell in correlation with different fertility population.Scientific reports · 2021Article
- Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era.Frontiers in genetics · 2019Review
- Landscape of alternative splicing in Capra_hircus.Scientific reports · 2018Article
- Efficient process development of recombinant human granulocyte colony-stimulating factor (rh-GCSF) production in Escherichia coli.Iranian biomedical journal · 2015Article
- Oviduct-specific expression of human neutrophil defensin 4 in lentivirally generated transgenic chickens.PloS one · 2015Article
- Sequencing and automated whole-genome optical mapping of the genome of a domestic goat (Capra hircus).Nature biotechnology · 2013Article
- Use of transgenic animals in biotechnology: prospects and problems.Acta naturae · 2013Article
- Transgenesis applied to goat: current applications and ongoing research.Transgenic research · 2012Review
- A 3,387 bp 5'-flanking sequence of the goat alpha-S1-casein gene provides correct tissue-specific expression of human granulocyte colony-stimulating factor (hG-CSF) in the mammary gland of transgenic mice.Transgenic research · 2012Article
- Laparoscopy vs. laparotomy for embryo transfer to produce transgenic goats (Capra hircus).Journal of veterinary science · 2008Article
- Lactation performance of transgenic goats expressing recombinant human butyryl-cholinesterase in the milk.Transgenic research · 2008Article
- Transgenic animal bioreactors: a new line of defense against chemical weapons?Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Generation and phenotypic analysis of a transgenic line of rabbits secreting active recombinant human erythropoietin in the milk.Transgenic research · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
19 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.