ArticleJournal of muscle research and cell motility2000
Functional diversity between orthologous myosins with minimal sequence diversity.
Article in Journal of muscle research and cell motility, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 39 citations in OpenAlex.
- Functional and Structural Diversity of Acyl-coA Binding Proteins in Oil Crops.Frontiers in genetics · 2018Review
- Actomyosin interaction at low ATP concentrations.European biophysics journal : EBJ · 2017Article
- Autophagy impairment in muscle induces neuromuscular junction degeneration and precocious aging.Cell reports · 2014Article
- Transgenic mouse α- and β-cardiac myosins containing the R403Q mutation show isoform-dependent transient kinetic differences.The Journal of biological chemistry · 2013Article
- Ultrafast force-clamp spectroscopy of single molecules reveals load dependence of myosin working stroke.Nature methods · 2012Article
- Actomyosin kinetics of pure fast and slow rat myosin isoforms studied by in vitro motility assay approach.Experimental physiology · 2012Article
- Myosin light chain 3f attenuates age-induced decline in contractile velocity in MHC type II single muscle fibers.Aging cell · 2012Article
- Long-term resistance training improves force and unloaded shortening velocity of single muscle fibres of elderly women.European journal of applied physiology · 2008Article
- Myosin as a potential redox-sensor: an in vitro study.Journal of muscle research and cell motility · 2008Article
- Skeletal muscle hypertrophy and structure and function of skeletal muscle fibres in male body builders.The Journal of physiology · 2006Article
- Two independent mechanical events in the interaction cycle of skeletal muscle myosin with actin.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Orthologous myosin isoforms and scaling of shortening velocity with body size in mouse, rat, rabbit and human muscles.The Journal of physiology · 2003Article
- Analysis of the myosins encoded in the recently completed Arabidopsis thaliana genome sequence.Genome biology · 2001Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To define the structural differences that are responsible for the functional diversity between orthologous sarcomeric myosins, we compared the rat and human beta/slow myosins. Functional comparison showed that rat beta/slow myosin has higher ATPase activity and moves actin filaments at higher speed in in vitro motility assay than human beta/slow myosin. Sequence analysis shows that the loop regions at the junctions of the 25 and 50 kDa domains (loop 1) and the 50 and 20 kDa domains (loop 2), which have been implicated in determining functional diversity of myosin heavy chains, are essentially identical in the two orthologs. There are only 14 non-conservative substitutions in the two myosin heavy chains, three of which are located in the secondary actin-binding loop and flanking regions and others correspond to residues so far not assigned a functional role, including two residues in the proximal S2 domain. Interestingly, in some of these positions the rat beta/slow myosin heavy chain has the same residues found in human cardiac alpha myosin, a fast-type myosin, and fast skeletal myosins. These observations indicate that functional and structural analysis of myosin orthologs with limited sequence diversity can provide useful clues to identify amino acid residues involved in modulating myosin function.
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