ArticleJournal of muscle research and cell motility2000
Actomyosin interactions in a novel single muscle fiber in vitro motility assay.
Article in Journal of muscle research and cell motility, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Relationship between in vivo muscle force at different speeds of isokinetic movements and myosin isoform expression in men and women.European journal of applied physiology · 2003Trial
- Chaperone co-inducer BGP-15 mitigates early contractile dysfunction of the soleus muscle in a rat ICU model.Acta physiologica (Oxford, England) · 2020Article
- Sarcopenia: Aging-Related Loss of Muscle Mass and Function.Physiological reviews · 2019 · on this mapReview
- Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle?International journal of molecular sciences · 2018Review
- Aberrant post-translational modifications compromise human myosin motor function in old age.Aging cell · 2015Article
- Poorly understood aspects of striated muscle contraction.BioMed research international · 2015Review
- Motor protein function in skeletal abdominal muscle of cachectic cancer patients.Journal of cellular and molecular medicine · 2014Article
- Evolving concepts on the age-related changes in "muscle quality".Journal of cachexia, sarcopenia and muscle · 2012Article
- Micromechanical thermal assays of Ca2+-regulated thin-filament function and modulation by hypertrophic cardiomyopathy mutants of human cardiac troponin.Journal of biomedicine & biotechnology · 2012Article
- Disrupted myosin cross-bridge cycling kinetics triggers muscle weakness in nebulin-related myopathy.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Force-generating capacity of human myosin isoforms extracted from single muscle fibre segments.The Journal of physiology · 2010Article
- Microarchitecture is severely compromised but motor protein function is preserved in dystrophic mdx skeletal muscle.Biophysical journal · 2010Article
- Adaptation by alternative RNA splicing of slow troponin T isoforms in type 1 but not type 2 Charcot-Marie-Tooth disease.American journal of physiology. Cell physiology · 2008Article
- Defective regulation of contractile function in muscle fibres carrying an E41K beta-tropomyosin mutation.The Journal of physiology · 2008Article
- Unloaded speed of shortening in voltage-clamped intact skeletal muscle fibers from wt, mdx, and transgenic minidystrophin mice using a novel high-speed acquisition system.Biophysical journal · 2008Article
- Skeletal muscle contractile protein function is preserved in human heart failure.Journal of applied physiology (Bethesda, Md. : 1985) · 2008Article
- Effects of a R133W beta-tropomyosin mutation on regulation of muscle contraction in single human muscle fibres.The Journal of physiology · 2007Article
- Muscle cell and motor protein function in patients with a IIa myosin missense mutation (Glu-706 to Lys).Neuromuscular disorders : NMD · 2006Article
- Regulatory proteins alter nucleotide binding to acto-myosin of sliding filaments in motility assays.Biophysical journal · 2003Article
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Authors and funding
2 authors.
Funding
Abstract
A novel in vitro motility assay has been developed to study the actomyosin interaction, in which the molecular motor protein myosin has been extracted and immobilized directly from 2-4 mm single rat skeletal muscle fiber segments. This method study was carried out to investigate: (1) the amount of myofibrillar proteins extracted from the fiber segment; (2) the effects of temperature on the speed at which actin is propelled by fast and slow myosin; and (3) the effects of myosin isoform expression on motility speed. Approximately 80% of the myosin and myosin-associated proteins were extracted from the fiber segments. while no significant extraction was shown of the thin filament proteins. Fluorescently labeled actin filaments moved with constant speed in a bi-directional motion over the high-density myosin region in the experimental chamber, and motility speed was highly dependent on the myosin heavy chain (MyHC) isoform extracted. At 25 degrees C, significant (P < 0.001) differences in motility speed were obtained between type I (1.31 +/- 0.23 microm/s, n = 11) and IIxb (5.81 +/- 0.35 microm/s, n = 6), or llb (6.07 +/- 0.33 microm/s, n = 8) MyHC isoforms. The motility speed and maximum velocity of unloaded shortening (V0) in single fibers were well correlated, indicating that filament speed is a good molecular analogue to contractile speed at the fiber level. The effects of temperature on filament motility speed were analyzed from 10 to 35 degrees C. The Q10 values, calculated in the 10-25 degrees C temperature range, differed between slow (4.20) and fast (2.38) myosin. In conclusion, this in vitro motility assay offers a unique possibility to compare the regulatory and modulatory influence of myosin isoforms and thin filament proteins on shortening velocity, at the cellular and molecular level in the same muscle fiber.
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Registered trials
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