ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2000
Comparative analysis of DNA methylation in tobacco heterochromatic sequences.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
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- Unraveling the DNA Methylation in the rDNA Foci in Mutagen-InducedInternational journal of molecular sciences · 2022Article
- Zebularine induces enzymatic DNA-protein crosslinks in 45S rDNA heterochromatin of Arabidopsis nuclei.Nucleic acids research · 2022Article
- Large-scale survey reveals pervasiveness and potential function of endogenous geminiviral sequences in plants.Virus evolution · 2020Article
- Remarkable variation of ribosomal DNA organization and copy number in gnetophytes, a distinct lineage of gymnosperms.Annals of botany · 2019Article
- Next generation sequencing analysis reveals a relationship between rDNA unit diversity and locus number in Nicotiana diploids.BMC genomics · 2012Article
- Independent, rapid and targeted loss of highly repetitive DNA in natural and synthetic allopolyploids of Nicotiana tabacum.PloS one · 2012Article
- Hypomethylating drugs efficiently decrease cytosine methylation in telomeric DNA and activate telomerase without affecting telomere lengths in tobacco cells.Plant molecular biology · 2011Article
- Inhibition of SAH-hydrolase activity during seed germination leads to deregulation of flowering genes and altered flower morphology in tobacco.Molecular genetics and genomics : MGG · 2011Article
- Similar patterns of rDNA evolution in synthetic and recently formed natural populations of Tragopogon (Asteraceae) allotetraploids.BMC evolutionary biology · 2010Article
- Structure and epigenetics of nucleoli in comparison with non-nucleolar compartments.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Review
- Cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic States in Arabidopsis.The Plant cell · 2010Article
- Linkage of 35S and 5S rRNA genes in Artemisia (family Asteraceae): first evidence from angiosperms.Chromosoma · 2009Article
- Chromatin: linking structure and function in the nucleolus.Chromosoma · 2009Review
- Dedifferentiation of tobacco cells is associated with ribosomal RNA gene hypomethylation, increased transcription, and chromatin alterations.Plant physiology · 2005Article
- DNA hypomethylation in 5-azacytidine-induced early-flowering lines of flax.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Dynamic changes in the distribution of a satellite homologous to intergenic 26-18S rDNA spacer in the evolution of Nicotiana.Genetics · 2004Article
- Studies on the effects of a flanking repetitive sequence on the expression of single-copy transgenes in Nicotiana sylvestris and in N. sylvestris-N. tomentosiformis hybrids.Plant molecular biology · 2003Article
- Divergence of satellite DNA and interspersion of dispersed repeats in the genome of the wild beet Beta procumbens.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Article
- Methylation of a euchromatin-heterochromatin transition region in Arabidopsis thaliana chromosome 5 left arm.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2002Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosine methylation levels and susceptibility to drug-induced hypomethylation have been studied in several Nicotiana tabacum (tobacco) DNA repetitive sequences. It has been shown using HapII, MspI, BamHI and Sau3AI methylation-sensitive restriction enzymes that the degree of 5'-mCmCG-3' methylation varied significantly between different repeats. There were almost saturation levels of 5-methylcytosine at the inner (3') cytosine position and variable degrees of methylation at the outer (5') cytosine at the enzyme recognition sites. The non-transcribed high copy satellite sequences (HRS60, GRS) displayed significant heterogeneity in methylation of their basic units while middle repetitive sequences (R8.1, GRD5, 5S rDNA) were more uniformly modified at both cytosine residues. Dihydroxypropyladenine (DHPA) treatment, which is thought to reduce DNA methyltransferase activity by increasing S-adenosylhomocysteine levels, resulted in extensive demethylation of the outer cytosine in all repeats, and the partial hypomethylation of cytosines at the inner positions in less densely methylated repeats such as HRS60 and GRS. The results suggest that hypomethylation of 5'-mCmCG-3' sites with DHPA is a gradual non-random process proceeding in the direction mCmCG-->CmCG-->CCG. The 18S-5.8S-25S rDNA was remarkably hypomethylated relative to the 5S rDNA at all restriction sites studied. Fluorescence in-situ hybridization showed that DNA decondensation within and between the 18S-5.8S-25S and 5S rDNA loci was variable in different nuclei. All nuclei had condensed and decondensed sequence. The chromatin of 18S-5.8S-25S rDNA was more readily digested with micrococcal nuclease than the 5S rDNA suggesting that the overall levels of decondensation were higher for 18S-5.8S-25S rDNA. Variable decondensation patterns within and between loci were also observed for GRS and HRS60. Cytosine methylation of the tobacco repeats is discussed with respect to transcription, overall levels of condensation and overall structure.
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