ArticleJournal of bacteriology2000
Nonspecific adherence by Actinobacillus actinomycetemcomitans requires genes widespread in bacteria and archaea.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers, 1 of them a synthesis that pooled it.
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Who cites it
109 citing papers in PubMed, 1 synthesis or guideline pooled it, 219 citations in OpenAlex.
- Acne and the cutaneous microbiome: A systematic review of mechanisms and implications for treatments.Journal of the European Academy of Dermatology and Venereology : JEADV · 2025Pooled it
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- ISAntibiotics (Basel, Switzerland) · 2025Article
- The structure of the Tad pilus alignment complex reveals a periplasmic conduit for pilus extension.Nature communications · 2025Article
- Biofilms and oral health: nanotechnology for biofilm control.Discover nano · 2025Review
- Tad pili with adaptable tips mediate contact-dependent killing during bacterial predation.Nature communications · 2025Article
- Prokaryotic morphological features and maintenance activities governed by seasonal productivity conditions.FEMS microbiology ecology · 2024Article
- Bidirectional pilus processing in the Tad pilus system motor CpaF.Nature communications · 2024Article
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- Tad pili contribute to the virulence and biofilm formation of virulentFrontiers in cellular and infection microbiology · 2024Article
- Role of biofilms in antimicrobial resistance of the bacterial bovine respiratory disease complex.Frontiers in veterinary science · 2024Review
- Assembly mechanism of a Tad secretion system secretin-pilotin complex.Nature communications · 2023Article
- Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask).Microbiology (Reading, England) · 2023Review
- Bacterial diet modulates tamoxifen-induced death via host fatty acid metabolism.Nature communications · 2022Article
- Complete Genome Sequence of Aggregatibacter actinomycetemcomitans Strain CU1000N.Microbiology resource announcements · 2022Article
- Genotype-phenotype correlations within theFrontiers in microbiology · 2022Article
- The Flp type IV pilus operon ofFrontiers in cellular and infection microbiology · 2022Article
- Signs of biofilm formation in the genome ofSaudi journal of biological sciences · 2021Article
- A comprehensive history of motility and Archaellation in Archaea.FEMS microbes · 2021Article
49 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gram-negative coccobacillus, Actinobacillus actinomycetemcomitans, is the putative agent for localized juvenile periodontitis, a particularly destructive form of periodontal disease in adolescents. This bacterium has also been isolated from a variety of other infections, notably endocarditis. Fresh clinical isolates of A. actinomycetemcomitans form tenacious biofilms, a property likely to be critical for colonization of teeth and other surfaces. Here we report the identification of a locus of seven genes required for nonspecific adherence of A. actinomycetemcomitans to surfaces. The recently developed transposon IS903phikan was used to isolate mutants of the rough clinical isolate CU1000 that are defective in tight adherence to surfaces (Tad(-)). Unlike wild-type cells, Tad(-) mutant cells adhere poorly to surfaces, fail to form large autoaggregates, and lack long, bundled fibrils. Nucleotide sequencing and genetic complementation analysis revealed a 6.7-kb region of the genome with seven adjacent genes (tadABCDEFG) required for tight adherence. The predicted TadA polypeptide is similar to VirB11, an ATPase involved in macromolecular transport. The predicted amino acid sequences of the other Tad polypeptides indicate membrane localization but no obvious functions. We suggest that the tad genes are involved in secretion of factors required for tight adherence of A. actinomycetemcomitans. Remarkably, complete and highly conserved tad gene clusters are present in the genomes of the bubonic plague bacillus Yersinia pestis and the human and animal pathogen Pasteurella multocida. Partial tad loci also occur in strikingly diverse Bacteria and Archaea. Our results show that the tad genes are required for tight adherence of A. actinomycetemcomitans to surfaces and are therefore likely to be essential for colonization and pathogenesis. The occurrence of similar genes in a wide array of microorganisms indicates that they have important functions. We propose that tad-like genes have a significant role in microbial colonization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.