ArticleJournal of bacteriology2000
Cloning and characterization of the str operon and elongation factor Tu expression in Bacillus stearothermophilus.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Improved transformation efficiency inMicrobiology spectrum · 2023Article
- The torpedo effect in Bacillus subtilis: RNase J1 resolves stalled transcription complexes.The EMBO journal · 2020Article
- Light-inducible expression of translation factor EF-Tu during acclimation to strong light enhances the repair of photosystem II.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Oxidation of Translation Factor EF-Tu Inhibits the Repair of Photosystem II.Plant physiology · 2018Article
- Manipulating natural product biosynthetic pathways via DNA assembler.Current protocols in chemical biology · 2014Article
- Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold.ACS synthetic biology · 2013Article
- Heavy involvement of stringent transcription control depending on the adenine or guanine species of the transcription initiation site in glucose and pyruvate metabolism in Bacillus subtilis.Journal of bacteriology · 2010Article
- Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- YbxF, a protein associated with exponential-phase ribosomes in Bacillus subtilis.Journal of bacteriology · 2007Article
- Archaeal elongation factor 1alpha from Sulfolobus solfataricus interacts with the eubacterial antibiotic GE2270A.Extremophiles : life under extreme conditions · 2004Article
- Analysis, characterization, and loci of the tuf genes in lactobacillus and bifidobacterium species and their direct application for species identification.Applied and environmental microbiology · 2003Article
- Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.Journal of bacteriology · 2002Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The complete primary structure of the str operon of Bacillus stearothermophilus was determined. It was established that the operon is a five-gene transcriptional unit: 5'-ybxF (unknown function; homology to eukaryotic ribosomal protein L30)-rpsL (S12)-rpsG (S7)-fus (elongation factor G [EF-G])-tuf (elongation factor Tu [EF-Tu])-3'. The main operon promoter (strp) was mapped upstream of ybxF, and its strength was compared with the strength of the tuf-specific promoter (tufp) located in the fus-tuf intergenic region. The strength of the tufp region to initiate transcription is about 20-fold higher than that of the strp region, as determined in chloramphenicol acetyltransferase assays. Deletion mapping experiments revealed that the different strengths of the promoters are the consequence of a combined effect of oppositely acting cis elements, identified upstream of strp (an inhibitory region) and tufp (a stimulatory A/T-rich block). Our results suggest that the oppositely adjusted core promoters significantly contribute to the differential expression of the str operon genes, as monitored by the expression of EF-Tu and EF-G.
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