ArticleMolecular biology of the cell2000
Wpkci, encoding an altered form of PKCI, is conserved widely on the avian W chromosome and expressed in early female embryos: implication of its role in female sex determination.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 165 citations in OpenAlex.
- New insights into Müllerian duct differentiation provided by single cell transcriptomics in the chicken embryo.PLoS genetics · 2026Article
- Identification of Key Differentially Expressed Genes During Early Sex Determination in Chicken Embryos.International journal of molecular sciences · 2025Article
- A W chromosome-derived feminizing piRNA in pyralid moths demonstrates convergent evolution for primary sex determination signals in Lepidoptera.BMC biology · 2025Article
- Article
- Evolution and expression patterns of the neo-sex chromosomes of the crested ibis.Nature communications · 2024Article
- A novel qPCR-based technique for identifying avian sex: An illustration within embryonic craniofacial bone.Genesis (New York, N.Y. : 2000) · 2024Article
- Fast, accurate, and cost-effective poultry sex genotyping using real-time polymerase chain reaction.Frontiers in veterinary science · 2023Article
- Prediction of sex-determination mechanisms in avian primordial germ cells using RNA-seq analysis.Scientific reports · 2022Article
- Fourth Report on Chicken Genes and Chromosomes 2022.Cytogenetic and genome research · 2022Review
- The Female-Specific W Chromosomes of Birds Have Conserved Gene Contents but Are Not Feminized.Genes · 2020Article
- Logical modelling uncovers developmental constraints for primary sex determination of chicken gonads.Journal of the Royal Society, Interface · 2018Article
- Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells.Oncogene · 2017Article
- Avian W and mammalian Y chromosomes convergently retained dosage-sensitive regulators.Nature genetics · 2017Article
- Matrilineal inheritance of a key mediator of prenatal maternal effects.Proceedings. Biological sciences · 2016Article
- Evolutionary analysis of the female-specific avian W chromosome.Nature communications · 2015Article
- Crucial genes and pathways in chicken germ stem cell differentiation.The Journal of biological chemistry · 2015Article
- Chromosome engineering allows the efficient isolation of vertebrate neocentromeres.Developmental cell · 2013Article
- RNA sequencing reveals sexually dimorphic gene expression before gonadal differentiation in chicken and allows comprehensive annotation of the W-chromosome.Genome biology · 2013Article
- High-resolution mapping and transcriptional activity analysis of chicken centromere sequences on giant lampbrush chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- W chromosome expression responds to female-specific selection.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two W chromosome-linked cDNA clones, p5fm2 and p5fm3, were obtained from a subtracted (female minus male) cDNA library prepared from a mixture of undifferentiated gonads and mesonephroi of male or female 5-d (stages 26-28) chicken embryos. These two clones were demonstrated to be derived from the mRNA encoding an altered form of PKC inhibitor/interacting protein (PKCI), and its gene was named Wpkci. The Wpkci gene reiterated approximately 40 times tandemly and located at the nonheterochromatic end of the chicken W chromosome. The W linkage and the moderate reiteration of Wpkci were conserved widely in Carinatae birds. The chicken PKCI gene, chPKCI, was shown to be a single-copy gene located near the centromere on the long arm of the Z chromosome. Deduced amino acid sequences of Wpkci and chPKCI showed approximately 65% identity. In the deduced sequence of Wpkci, the HIT motif, which is essential for PKCI function, was absent, but the alpha-helix region, which was conserved among the PKCI family, and a unique Leu- and Arg-rich region, were present. Transcripts from both Wpkci and chPKCI genes were present at significantly higher levels in 3- to 6-d (stages 20-29) embryos. These transcripts were detected in several embryonic tissues, including undifferentiated left and right gonads. When the green fluorescent protein-fused form of Wpkci was expressed in male chicken embryonic fibroblast, it was located almost exclusively in the nucleus. A model is presented suggesting that Wpkci may be involved in triggering the differentiation of ovary by interfering with PKCI function or by exhibiting its unique function in the nuclei of early female embryos.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.