ArticleMolecular biology of the cell2000
Vimentin filaments in fibroblasts are a reservoir for SNAP23, a component of the membrane fusion machinery.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 74 citations in OpenAlex.
- Pathophysiological Role of Vimentin Intermediate Filaments in Lung Diseases.Frontiers in cell and developmental biology · 2022Review
- VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation.Frontiers in cell and developmental biology · 2020Article
- ADP-ribosylation Factor-related Protein 1 Interacts with NS5A and Regulates Hepatitis C Virus Propagation.Scientific reports · 2016Article
- Review
- The neuronal porosome complex in health and disease.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Porosome: a membrane microdomain acting as the universal secretory portal in exocytosis.Discoveries (Craiova, Romania) · 2014Review
- NeuronalJournal of molecular structure · 2014Article
- Vimentin mediates uptake of C3 exoenzyme.PloS one · 2014Article
- In vitro characterization of a chitosan skin regenerating template as a scaffold for cells cultivation.SpringerPlus · 2013Article
- Neuronal porosome proteome: Molecular dynamics and architecture.Journal of proteomics · 2012Article
- Ablation of vimentin results in defective steroidogenesis.Endocrinology · 2012Article
- Protein composition of immunoprecipitated synaptic ribbons.Journal of proteome research · 2012Article
- Synaptic vesicle-like lipidome of human cytomegalovirus virions reveals a role for SNARE machinery in virion egress.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Soluble CD44 interacts with intermediate filament protein vimentin on endothelial cell surface.PloS one · 2011Article
- New insights into molecular Ehrlichia chaffeensis-host interactions.Microbes and infection · 2010Review
- NF-kappaB functions in stromal fibroblasts to regulate early postnatal muscle development.The Journal of biological chemistry · 2010Article
- Atomic force microscopy: Unraveling the fundamental principles governing secretion and membrane fusion in cells.Ultramicroscopy · 2009Review
- Vimentin regulates scribble activity by protecting it from proteasomal degradation.Molecular biology of the cell · 2009Article
- Porosome: the secretory portal in cells.Biochemistry · 2009Review
- Pivotal advance: IgE accelerates in vitro development of mast cells and modifies their phenotype.Journal of leukocyte biology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Soluble N-ethyl maleimide-sensitive fusion protein attachment protein receptors (SNAREs) are core machinery for membrane fusion during intracellular vesicular transport. Synaptosome-associated protein of 23 kDa (SNAP23) is a target SNARE previously identified at the plasma membrane, where it is involved in exocytotic membrane fusion. Here we show that SNAP23 associates with vimentin filaments in a Triton X-100 insoluble fraction in fibroblasts in primary culture and HeLa cells. Upon treatment of human fibroblasts with N-ethyl-maleimide, SNAP23 dissociates from vimentin filaments and forms a protein complex with syntaxin 4, a plasma membrane SNARE. The vimentin-associated pool of SNAP23 can therefore be a reservoir, which would supply the plasma membrane fusion machinery, in fibroblasts. Our observation points to a yet unexplored role of intermediate filaments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.