ArticleMolecular biology of the cell2000
Identification of a new vertebrate nucleoporin, Nup188, with the use of a novel organelle trap assay.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 57 citations in OpenAlex.
- A checkpoint function for Nup98 in nuclear pore formation suggested by novel inhibitory nanobodies.The EMBO journal · 2024Article
- A rare cause of steroid-resistant nephrotic syndrome - a case report.Central-European journal of immunology · 2023Article
- A Boy with Sandestig-Stefanova Syndrome and Genital Abnormalities.Molecular syndromology · 2022Article
- The nuclear pore complex: understanding its function through structural insight.Nature reviews. Molecular cell biology · 2017Review
- HOXA repression is mediated by nucleoporin Nup93 assisted by its interactors Nup188 and Nup205.Epigenetics & chromatin · 2016Article
- Review
- The interaction of RNA helicase DDX3 with HIV-1 Rev-CRM1-RanGTP complex during the HIV replication cycle.PloS one · 2015Article
- In vivo analysis of human nucleoporin repeat domain interactions.Molecular biology of the cell · 2013Article
- Genome-wide patterns of natural variation reveal strong selective sweeps and ongoing genomic conflict in Drosophila mauritiana.Genome research · 2013Article
- The C-terminal domain of Nup93 is essential for assembly of the structural backbone of nuclear pore complexes.Molecular biology of the cell · 2012Article
- Article
- Quantitative proteomic analysis of A549 cells infected with human respiratory syncytial virus.Molecular & cellular proteomics : MCP · 2010Article
- The nuclear pore complex and nuclear transport.Cold Spring Harbor perspectives in biology · 2010Review
- Recurrent deletion of 9q34 in adult normal karyotype precursor B-cell acute lymphoblastic leukemia.Cancer genetics and cytogenetics · 2010Article
- Architectural nucleoporins Nup157/170 and Nup133 are structurally related and descend from a second ancestral element.The Journal of biological chemistry · 2009Article
- Nuclear pore disassembly from endoplasmic reticulum membranes promotes Ca2+ signalling competency.The Journal of physiology · 2008Article
- Centrin 2 localizes to the vertebrate nuclear pore and plays a role in mRNA and protein export.Molecular and cellular biology · 2008Article
- ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex.Molecular biology of the cell · 2005Article
- Nucleoporins as components of the nuclear pore complex core structure and Tpr as the architectural element of the nuclear basket.Molecular biology of the cell · 2004Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The study of the nuclear pore in vertebrates would benefit from a strategy to directly identify new nucleoporins and interactions between those nucleoporins. We have developed a novel two-step "organelle trap" assay involving affinity selection and in vitro pore assembly. In the first step, soluble proteins derived from Xenopus egg extracts are applied to a column containing a ligand of interest. The bound proteins are then tagged by biotinylation and eluted. In the second step, potential nucleoporins are selected for by virtue of their ability to assemble into annulate lamellae, a cytoplasmic mimic of nuclear pores. The incorporated proteins are then recognized by their biotin tag. Here we use the lectin wheat germ agglutinin (WGA) as ligand; WGA inhibits nuclear transport and has been shown to directly bind three known nucleoporins from Xenopus extract, Nup62, Nup98, and Nup214, all of which contain N-acetylglucosamine residues. Under reduced-stringency conditions, three additional proteins bind to WGA-Sepharose and are revealed by the organelle trap assay. We identified all three as partner nucleoporins. Two were discovered to be Xenopus Nup93 and Nup205. The third is a novel vertebrate nucleoporin, Nup188. This new vertebrate protein, Xenopus Nup188, exists in a complex with xNup93 and xNup205. The Nup93-Nup188-Nup205 complex does not bind directly to WGA but binds indirectly via the N-acetylglucosamine-modified nucleoporins. A gene encoding human Nup188 was also identified. The discovery of vertebrate Nup188, related to a yeast nucleoporin, and its novel protein-protein interactions illustrates the power of the two-step organelle trap assay and identifies new building blocks for constructing the nuclear pore.
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