ArticleMolecular biology of the cell2000
Forced expression of keratin 16 alters the adhesion, differentiation, and migration of mouse skin keratinocytes.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- TGF-beta coordinates changes in Keratin gene expression during complex tissue regeneration.Scientific reports · 2025Article
- Shifts in keratin isoform expression activate motility signals during wound healing.Developmental cell · 2024Article
- Keratin isoform shifts modulate motility signals during wound healing.bioRxiv : the preprint server for biology · 2024Article
- miR-204-3p downregulates KRT16 and promotes corneal repair in tree shrew fungal keratitis model.American journal of translational research · 2022Article
- Emerging Insights into Keratin 16 Expression during Metastatic Progression of Breast Cancer.Cancers · 2021Article
- Foxn1 in Skin Development, Homeostasis and Wound Healing.International journal of molecular sciences · 2018Review
- A shift from papillary to reticular fibroblasts enables tumour-stroma interaction and invasion.British journal of cancer · 2018Article
- Foxn1 Transcription Factor Regulates Wound Healing of Skin through Promoting Epithelial-Mesenchymal Transition.PloS one · 2016Article
- Vimentin is necessary for colony growth of human diploid keratinocytes.Histochemistry and cell biology · 2015Article
- Downregulation of STRA6 expression in epidermal keratinocytes leads to hyperproliferation-associated differentiation in both in vitro and in vivo skin models.The Journal of investigative dermatology · 2014Article
- Bone morphogenetic protein signaling suppresses wound-induced skin repair by inhibiting keratinocyte proliferation and migration.The Journal of investigative dermatology · 2014Article
- Keratins mediate localization of hemidesmosomes and repress cell motility.The Journal of investigative dermatology · 2013Article
- Novel function of keratins 5 and 14 in proliferation and differentiation of stratified epithelial cells.Molecular biology of the cell · 2011Article
- Differential keratin expression during epiboly in a wound model of bioengineered skin and in human chronic wounds.The international journal of lower extremity wounds · 2011Article
- Structure and functions of keratin proteins in simple, stratified, keratinized and cornified epithelia.Journal of anatomy · 2009Review
- Keratin 17 modulates hair follicle cycling in a TNFalpha-dependent fashion.Genes & development · 2006Article
- Elimination of epiplakin by gene targeting results in acceleration of keratinocyte migration in mice.Molecular and cellular biology · 2006Article
- CXCR4 knockdown by small interfering RNA abrogates breast tumor growth in vivo.Cancer gene therapy · 2005Article
- Overcoming functional redundancy to elicit pachyonychia congenita-like nail lesions in transgenic mice.Molecular and cellular biology · 2005Article
- The nonhelical tail domain of keratin 14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro.The Journal of cell biology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Injury to the skin results in an induction of keratins K6, K16, and K17 concomitant with activation of keratinocytes for reepithelialization. Forced expression of human K16 in skin epithelia of transgenic mice causes a phenotype that mimics several aspects of keratinocyte activation. Two types of transgenic keratinocytes, with forced expression of either human K16 or a K16-C14 chimeric cDNA, were analyzed in primary culture to assess the impact of K16 expression at a cellular level. High K16-C14-expressing and low K16-expressing transgenic keratinocytes behave similar to wild type in all aspects tested. In contrast, high K16-expressing transgenic keratinocytes show alterations in plating efficiency and calcium-induced differentiation, but proliferate normally. Migration of keratinocytes is reduced in K16 transgenic skin explants compared with controls. Finally, a subset of high K16-expressing transgenic keratinocytes develops major changes in the organization of keratin filaments in a time- and calcium concentration-dependent manner. These changes coincide with alterations in keratin content while the steady-state levels of K16 protein remain stable. We conclude that forced expression of K16 in progenitor skin keratinocytes directly impacts properties such as adhesion, differentiation, and migration, and that these effects depend upon determinants contained within its carboxy terminus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.