ArticlePlant physiology2000
Antisense suppression of 2-cysteine peroxiredoxin in Arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 178 citations in OpenAlex.
- Response of photosynthetic characteristics and antioxidant system in the leaves of safflower to NaCl and NaHCOPlant cell reports · 2024Article
- The ascorbate-glutathione cycle coming of age.Journal of experimental botany · 2024Review
- Using chlorophyll fluorescence parameters and antioxidant enzyme activity to assess drought tolerance of spring wheat.Photosynthetica · 2024Article
- Reduced GSH Acts as a Metabolic Cue of OPDA Signaling in Coregulating Photosynthesis and Defense Activation under Stress.Plants (Basel, Switzerland) · 2023Article
- Molecular Regulation and Evolution of Redox Homeostasis in Photosynthetic Machinery.Antioxidants (Basel, Switzerland) · 2022Review
- A chloroplast redox relay adapts plastid metabolism to light and affects cytosolic protein quality control.Plant physiology · 2021Article
- Label-Free Quantitative Proteomics Analysis in Susceptible and ResistantMicroorganisms · 2021Article
- Effect of various abiotic stressors on some biochemical indices of Lepidium sativum plants.Scientific reports · 2020Article
- CYP20-3 deglutathionylates 2-CysPRX A and suppresses peroxide detoxification during heat stress.Life science alliance · 2020Article
- Overexpression ofInternational journal of molecular sciences · 2020Article
- Evaluation of the effects of humic acids on maize root architecture by label-free proteomics analysis.Scientific reports · 2019Article
- Article
- Specificity versus redundancy in the RAP2.4 transcription factor family of Arabidopsis thaliana: transcriptional regulation of genes for chloroplast peroxidases.BMC plant biology · 2017Article
- The Plant Immunity Regulating F-Box ProteinFrontiers in plant science · 2017Article
- Cold regulation of plastid ascorbate peroxidases serves as a priming hub controlling ROS signaling in Arabidopsis thaliana.BMC plant biology · 2016Article
- Regions of the bread wheat D genome associated with variation in key photosynthesis traits and shoot biomass under both well watered and water deficient conditions.Journal of applied genetics · 2016Article
- Interactions between 2-Cys peroxiredoxins and ascorbate in autophagosome formation during the heat stress response in Solanum lycopersicum.Journal of experimental botany · 2016Article
- Natural Variation of Cold Deacclimation Correlates with Variation of Cold-Acclimation of the Plastid Antioxidant System in Arabidopsis thaliana Accessions.Frontiers in plant science · 2016Article
- Thiol-Based Peroxidases and Ascorbate Peroxidases: Why Plants Rely on Multiple Peroxidase Systems in the Photosynthesizing Chloroplast?Molecules and cells · 2016Review
- Site-directed mutagenesis substituting cysteine for serine in 2-Cys peroxiredoxin (2-Cys Prx A) of Arabidopsis thaliana effectively improves its peroxidase and chaperone functions.Annals of botany · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to characterize the effect of decreased 2-cysteine peroxiredoxin (2-CP) on the leaf anti-oxidative system in Arabidopsis. At three stages of leaf development, two lines of transgenic Arabidopsis mutants with decreased contents of chloroplast 2-CP were compared with wild type and a control line transformed with an empty vector. Glutathione contents and redox state were similar in all plants, and no changes in transcript levels for enzymes involved in glutathione metabolism were observed. Transcript levels for chloroplastic glutathione peroxidase were much lower than those for 2-CP, and both cytosolic and chloroplastic glutathione peroxidase were not increased in the mutants. In contrast, the foliar ascorbate pool was more oxidized in the mutants, although the difference decreased with plant age. The activities of thylakoid and stromal ascorbate peroxidase and particularly monodehydroascorbate reductase were increased as were transcripts for these enzymes. No change in dehydroascorbate reductase activity was observed, and effects on transcript abundance for glutathione reductase, catalase, and superoxide dismutase were slight or absent. The results demonstrate that 2-CP forms an integral part of the anti-oxidant network of chloroplasts and is functionally interconnected with other defense systems. Suppression of 2-CP leads to increased expression of other anti-oxidative genes possibly mediated by increased oxidation state of the leaf ascorbate pool.
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