ArticleMolecular and cellular biology2000
Participation of the C-terminal domain of RNA polymerase II in exon definition during pre-mRNA splicing.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 76 citations in OpenAlex.
- Co-transcriptional mRNP formation is coordinated within a molecular mRNP packaging station in S. cerevisiae.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- Diversity in TAF proteomics: consequences for cellular differentiation and migration.International journal of molecular sciences · 2014Review
- RNA polymerase II C-terminal domain: Tethering transcription to transcript and template.Chemical reviews · 2013Review
- Review
- The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex.Genes & development · 2011Article
- Updating the CTD Story: From Tail to Epic.Genetics research international · 2011Article
- Efficient transcription through an intron requires the binding of an Sm-type U1 snRNP with intact stem loop II to the splice donor.Nucleic acids research · 2010Article
- The neurofibromatosis type I pre-mRNA is a novel target of CELF protein-mediated splicing regulation.Nucleic acids research · 2010Article
- Connections between alternative transcription and alternative splicing in mammals.Genome biology and evolution · 2010Article
- Rates of in situ transcription and splicing in large human genes.Nature structural & molecular biology · 2009Article
- Polyadenylation releases mRNA from RNA polymerase II in a process that is licensed by splicing.RNA (New York, N.Y.) · 2009Article
- The Carboxyl-terminal Domain of RNA Polymerase II Is Not Sufficient to Enhance the Efficiency of Pre-mRNA Capping or Splicing in the Context of a Different Polymerase.The Journal of biological chemistry · 2009Article
- Repression of prespliceosome complex formation at two distinct steps by Fox-1/Fox-2 proteins.Molecular and cellular biology · 2008Article
- Functional coupling of last-intron splicing and 3'-end processing to transcription in vitro: the poly(A) signal couples to splicing before committing to cleavage.Molecular and cellular biology · 2008Article
- Transcriptional activity of androgen receptor is modulated by two RNA splicing factors, PSF and p54nrb.Molecular and cellular biology · 2007Article
- Human transcription elongation factor CA150 localizes to splicing factor-rich nuclear speckles and assembles transcription and splicing components into complexes through its amino and carboxyl regions.Molecular and cellular biology · 2006Article
- Splicing speckles are not reservoirs of RNA polymerase II, but contain an inactive form, phosphorylated on serine2 residues of the C-terminal domain.Molecular biology of the cell · 2006Article
- Distal regulation of alternative splicing by splicing enhancer in equine beta-casein intron 1.RNA (New York, N.Y.) · 2006Article
- A subset of nuclear receptor coregulators act as coupling proteins during synthesis and maturation of RNA transcripts.Molecular and cellular biology · 2005Review
- The C-terminal domain of RNA polymerase II functions as a phosphorylation-dependent splicing activator in a heterologous protein.Molecular and cellular biology · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Interaction between transcription and pre-mRNA processing via binding of polymerase II (Pol II) to factors involved in capping, splicing, and polyadenylation has recently been demonstrated. The C-terminal domain (CTD), a highly phosphorylated repeat sequence of the largest subunit of Pol II, has been implicated in this interaction because deletion of this domain affects downstream RNA processing events and because it is the binding site for numerous processing factors. Here we show that recombinant CTD, free of other components of Pol II, activated in vitro splicing and assembly of the spliceosome in nuclear extracts if, and only if, the assayed precursor RNA was recognized via exon definition, i.e., if the substrates contained complete exons with both 3' and 5' splice sites. Furthermore, depletion of intact Pol II inactivated splicing of this set of precursor RNAs and addition of recombinant CTD restored activity. The added recombinant CTD was quickly hyper- and hypophosphorylated in extract, became associated with the precursor RNA, and stimulated the association of U1 snRNPs but not ASF/SF2 with substrate RNA. These observations suggest that the mode of interaction between the CTD and splicing factors is integrally tied to exon definition and the mechanism whereby distal exons can be recognized and brought into juxtaposition during assembly of the spliceosome.
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