ArticleMolecular and cellular biology2000
Function of the ski4p (Csl4p) and Ski7p proteins in 3'-to-5' degradation of mRNA.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed, 185 citations in OpenAlex.
- Phosphorylation Patterns of Preribosomal Proteins Associated with the RNA Exosome.Journal of proteome research · 2026Article
- Yeast elongation factor homolog New1 protects a subset of mRNAs from degradation by no-go decay.Nucleic acids research · 2026Article
- New insights into nuclear import and nucleolar localization of yeast RNA exosome subunits.Molecular biology of the cell · 2025Article
- The ribosome as a platform to coordinate mRNA decay.Nucleic acids research · 2025Review
- Polyadenylated versions of small non-coding RNAs inMicrobial cell (Graz, Austria) · 2024Article
- Pervasive mRNA uridylation in fission yeast is catalysed by both Cid1 and Cid16 terminal uridyltransferases.PloS one · 2023Article
- Article
- A yeast model for trichohepatoenteric syndrome suggests strong loss of Ski2 function in most causative mutations.microPublication biology · 2022Article
- LISTERIN E3 Ubiquitin Ligase and Ribosome-Associated Quality Control (RQC) Mechanism.Molecular neurobiology · 2021Review
- Zebrafish Ski7 tunes RNA levels during the oocyte-to-embryo transition.PLoS genetics · 2021Article
- Suppressors of mRNA Decapping Defects Restore Growth Without Major Effects on mRNA Decay Rates or Abundance.Genetics · 2020Article
- Origin, conservation, and loss of alternative splicing events that diversify the proteome in Saccharomycotina budding yeasts.RNA (New York, N.Y.) · 2020Article
- RNA processing machineries in Archaea: the 5'-3' exoribonuclease aRNase J of the β-CASP family is engaged specifically with the helicase ASH-Ski2 and the 3'-5' exoribonucleolytic RNA exosome machinery.Nucleic acids research · 2020Article
- Mammalian RNA Decay Pathways Are Highly Specialized and Widely Linked to Translation.Molecular cell · 2020Article
- The RNA Exosome and Human Disease.Methods in molecular biology (Clifton, N.J.) · 2020Review
- In Vitro Characterization of the Activity of the Mammalian RNA Exosome on mRNAs in Ribosomal Translation Complexes.Methods in molecular biology (Clifton, N.J.) · 2020Article
- A specialised SKI complex assists the cytoplasmic RNA exosome in the absence of direct association with ribosomes.The EMBO journal · 2019Article
- Pelota-interacting G protein Hbs1 is required for spermatogenesis in Drosophila.Scientific reports · 2019Article
- The Pat1-Lsm Complex Stabilizes ATG mRNA during Nitrogen Starvation-Induced Autophagy.Molecular cell · 2019Article
- The Yeast DNA Damage Checkpoint Kinase Rad53 Targets the Exoribonuclease, Xrn1.G3 (Bethesda, Md.) · 2018Article
33 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of two general pathways of mRNA decay in the yeast Saccharomyces cerevisiae occurs by deadenylation followed by 3'-to-5' degradation of the mRNA body. Previous results have shown that this degradation requires components of the exosome and the Ski2p, Ski3p, and Ski8p proteins, which were originally identified due to their superkiller phenotype. In this work, we demonstrate that deletion of the SKI7 gene, which encodes a putative GTPase, also causes a defect in 3'-to-5' degradation of mRNA. Deletion of SKI7, like deletion of SKI2, SKI3, or SKI8, does not affect various RNA-processing reactions of the exosome. In addition, we show that a mutation in the SKI4 gene also causes a defect in 3'-to-5' mRNA degradation. We show that the SKI4 gene is identical to the CSL4 gene, which encodes a core component of the exosome. Interestingly, the ski4-1 allele contains a point mutation resulting in a mutation in the putative RNA binding domain of the Csl4p protein. This point mutation strongly affects mRNA degradation without affecting exosome function in rRNA or snRNA processing, 5' externally transcribed spacer (ETS) degradation, or viability. In contrast, the csl4-1 allele of the same gene affects rRNA processing but not 3'-to-5' mRNA degradation. We identify csl4-1 as resulting from a partial-loss-of-function mutation in the promoter of the CSL4 gene. These data indicate that the distinct functions of the exosome can be separated genetically and suggest that the RNA binding domain of Csl4p may have a specific function in mRNA degradation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.