ArticleMolecular and cellular biology2000
Mechanism of promoter melting by the xeroderma pigmentosum complementation group B helicase of transcription factor IIH revealed by protein-DNA photo-cross-linking.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Excessive MYC-topoisome activity triggers acute DNA damage, MYC degradation, and replacement by a p53-topoisome.Molecular cell · 2024Article
- Structural polymorphism of the PH domain in TFIIH.Bioscience reports · 2023Article
- Selection signatures in tropical cattle are enriched for promoter and coding regions and reveal missense mutations in the damage response gene HELB.Genetics, selection, evolution : GSE · 2020Article
- Structural basis of transcription initiation by RNA polymerase II.Nature reviews. Molecular cell biology · 2015Review
- In TFIIH, XPD helicase is exclusively devoted to DNA repair.PLoS biology · 2014Article
- Architecture of the RNA polymerase II preinitiation complex and mechanism of ATP-dependent promoter opening.Nature structural & molecular biology · 2012Article
- Individual basepair stability of DNA and RNA studied by NMR-detected solvent exchange.Biophysical journal · 2012Article
- TFIIH: when transcription met DNA repair.Nature reviews. Molecular cell biology · 2012Review
- Subunit architecture of general transcription factor TFIIH.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Mechanism of start site selection by RNA polymerase II: interplay between TFIIB and Ssl2/XPB helicase subunit of TFIIH.The Journal of biological chemistry · 2012Article
- TFIIH is an elongation factor of RNA polymerase I.Nucleic acids research · 2012Article
- Review
- Use of site-specific protein-DNA photocrosslinking of purified complexes to analyze the topology of the RNA polymerase II transcription initiation complex.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Structural insight into the TFIIE-TFIIH interaction: TFIIE and p53 share the binding region on TFIIH.The EMBO journal · 2008Article
- Structural characterization of human general transcription factor TFIIF in solution.Protein science : a publication of the Protein Society · 2008Article
- The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex.Nature structural & molecular biology · 2007Article
- A DNA-tethered cleavage probe reveals the path for promoter DNA in the yeast preinitiation complex.Nature structural & molecular biology · 2006Article
- Structural perspective on mutations affecting the function of multisubunit RNA polymerases.Microbiology and molecular biology reviews : MMBR · 2006Review
- Functional dissection of the catalytic mechanism of mammalian RNA polymerase II.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2005Review
- Stimulation of the XPB ATP-dependent helicase by the beta subunit of TFIIE.Nucleic acids research · 2005Article
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7 authors.
Funding
Abstract
The p89/xeroderma pigmentosum complementation group B (XPB) ATPase-helicase of transcription factor IIH (TFIIH) is essential for promoter melting prior to transcription initiation by RNA polymerase II (RNAPII). By studying the topological organization of the initiation complex using site-specific protein-DNA photo-cross-linking, we have shown that p89/XPB makes promoter contacts both upstream and downstream of the initiation site. The upstream contact, which is in the region where promoter melting occurs (positions -9 to +2), requires tight DNA wrapping around RNAPII. The addition of hydrolyzable ATP tethers the template strand at positions -5 and +1 to RNAPII subunits. A mutation in p89/XPB found in a xeroderma pigmentosum patient impairs the ability of TFIIH to associate correctly with the complex and thereby melt promoter DNA. A model for open complex formation is proposed.
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