ArticleMolecular and cellular biology2000
cis- and trans-Acting determinants for translation of psbD mRNA in Chlamydomonas reinhardtii.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- A modular high-throughput approach for advancing synthetic biology in the chloroplast of Chlamydomonas.Nature plants · 2025Article
- Assembly and Repair of Photosystem II inPlants (Basel, Switzerland) · 2024Review
- Plant organellar RNA maturation.The Plant cell · 2023Review
- RNAStructuromeDB: A genome-wide database for RNA structural inference.Scientific reports · 2017Article
- Redox regulation of thylakoid protein kinases and photosynthetic gene expression.Antioxidants & redox signaling · 2013Review
- How to build functional thylakoid membranes: from plastid transcription to protein complex assembly.Planta · 2013Review
- Recruitment of a ribosomal release factor for light- and stress-dependent regulation of petB transcript stability in Arabidopsis chloroplasts.The Plant cell · 2011Article
- Function of chloroplast RNA-binding proteins.Cellular and molecular life sciences : CMLS · 2011Review
- The nucleus-encoded trans-acting factor MCA1 plays a critical role in the regulation of cytochrome f synthesis in Chlamydomonas chloroplasts.The Plant cell · 2011Article
- Red light and calmodulin regulate the expression of the psbA binding protein genes in Chlamydomonas reinhardtii.Plant & cell physiology · 2010Article
- Molecular identification and function of cis- and trans-acting determinants for petA transcript stability in Chlamydomonas reinhardtii chloroplasts.Molecular and cellular biology · 2008Article
- Article
- The nuclear-encoded factor HCF173 is involved in the initiation of translation of the psbA mRNA in Arabidopsis thaliana.The Plant cell · 2007Article
- Translation of chloroplast psbD mRNA in Chlamydomonas is controlled by a secondary RNA structure blocking the AUG start codon.Nucleic acids research · 2006Article
- Contribution of 5'- and 3'-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes.Molecular genetics and genomics : MGG · 2005Article
- NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii.The Plant cell · 2005Article
- Relationship between mRNA levels and protein accumulation in a chloroplast promoter-mutant of Chlamydomonas reinhardtii.Molecular genetics and genomics : MGG · 2005Article
- Chloroplast RNA processing and stability.Photosynthesis research · 2004Article
- Chloroplast RNA-binding proteins.Current genetics · 2003Review
- A role for initiation codon context in chloroplast translation.The Plant cell · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplast translation is mediated by nucleus-encoded factors that interact with distinct cis-acting RNA elements. A U-rich sequence within the 5' untranslated region of the psbD mRNA has previously been shown to be required for its translation in Chlamydomonas reinhardtii. By using UV cross-linking assays, we have identified a 40-kDa RNA binding protein, which binds to the wild-type psbD leader, but is unable to recognize a nonfunctional leader mutant lacking the U-rich motif. RNA binding is restored in a chloroplast cis-acting suppressor. The functions of several site-directed psbD leader mutants were analyzed with transgenic C. reinhardtii chloroplasts and the in vitro RNA binding assay. A clear correlation between photosynthetic activity and the capability to bind RNA by the 40-kDa protein was observed. Furthermore, the data obtained suggest that the poly(U) region serves as a molecular spacer between two previously characterized cis-acting elements, which are involved in RNA stabilization and translation. RNA-protein complex formation depends on the nuclear Nac2 gene product that is part of a protein complex required for the stabilization of the psbD mRNA. The sedimentation properties of the 40-kDa RNA binding protein suggest that it interacts directly with this Nac2 complex and, as a result, links processes of chloroplast RNA metabolism and translation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.