ArticleMolecular and cellular biology2000
Multiple mechanisms of suppression circumvent transcription defects in an RNA polymerase mutant.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- La-motif-dependent mRNA association with Slf1 promotes copper detoxification in yeast.RNA (New York, N.Y.) · 2012Article
- mRNA imprinting: Additional level in the regulation of gene expression.Cellular logistics · 2011Article
- Nucleocytoplasmic shuttling of the La motif-containing protein Sro9 might link its nuclear and cytoplasmic functions.RNA (New York, N.Y.) · 2010Article
- A comprehensive analysis of the La-motif protein superfamily.RNA (New York, N.Y.) · 2009Article
- C. elegans La-related protein, LARP-1, localizes to germline P bodies and attenuates Ras-MAPK signaling during oogenesis.RNA (New York, N.Y.) · 2008Article
- Genomewide recruitment analysis of Rpb4, a subunit of polymerase II in Saccharomyces cerevisiae, reveals its involvement in transcription elongation.Eukaryotic cell · 2008Article
- The RNA polymerase II subunit Rpb4p mediates decay of a specific class of mRNAs.Genes & development · 2005Article
- Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II.Nucleic acids research · 2005Article
- Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts.Nucleic acids research · 2003Article
- Rpb4p, a subunit of RNA polymerase II, mediates mRNA export during stress.Molecular biology of the cell · 2003Article
- Complete, 12-subunit RNA polymerase II at 4.1-A resolution: implications for the initiation of transcription.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Architecture of initiation-competent 12-subunit RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- La protein and its associated small nuclear and nucleolar precursor RNAs.Gene expression · 2002Review
- Conserved and divergent features of the structure and function of La and La-related proteins (LARPs).Biochimica et biophysica actaReview
- Conserved Functions of LARP1 Proteins in Eukaryotes.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Using a high-copy-number suppressor screen to obtain clues about the role of the yeast RNA polymerase II subunit RPB4 in transcription, we identified three suppressors of the temperature sensitivity resulting from deletion of the RPB4 gene (DeltaRPB4). One suppressor is Sro9p, a protein related to La protein, another is the nucleosporin Nsp1p, and the third is the RNA polymerase II subunit RPB7. Suppression by RPB7 was anticipated since its interaction with RPB4 is well established both in vitro and in vivo. We examined the effect of overexpression of each suppressor gene on transcription. Interestingly, suppression of the temperature-sensitive phenotype correlates with the correction of a characteristic transcription defect of this mutant: each suppressor restored the level of promoter-specific, basal transcription to wild-type levels. Examination of the effects of the suppressors on other in vivo transcription aberrations in DeltaRPB4 cells revealed significant amelioration of defects in certain inducible genes in Sro9p and RPB7, but not in Nsp1p, suppressor cells. Analysis of mRNA levels demonstrated that overexpression of each of the three suppressors minimally doubled the mRNA levels during stationary phase. However, the elevated mRNA levels in Sro9p suppressor cells appear to result from a combination of enhanced transcription and message stability. Taken together, these results demonstrate that these three proteins influence transcription and implicate Sro9p in both transcription and posttranscription events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.