ArticleMolecular and cellular biology2000
Identification of novel Saccharomyces cerevisiae proteins with nuclear export activity: cell cycle-regulated transcription factor ace2p shows cell cycle-independent nucleocytoplasmic shuttling.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources.iScience · 2023Article
- Article
- Rbs1, a new protein implicated in RNA polymerase III biogenesis in yeast Saccharomyces cerevisiae.Molecular and cellular biology · 2015Article
- The Rts1 regulatory subunit of PP2A phosphatase controls expression of the HO endonuclease via localization of the Ace2 transcription factor.The Journal of biological chemistry · 2014Article
- Mitotic exit and separation of mother and daughter cells.Genetics · 2012Review
- RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor.Molecular biology of the cell · 2012Article
- Mutations in the C-terminus of the conserved NDR kinase, Cbk1p of Saccharomyces cerevisiae, make the protein independent of upstream activators.Molecular genetics and genomics : MGG · 2010Article
- Hyphal chain formation in Candida albicans: Cdc28-Hgc1 phosphorylation of Efg1 represses cell separation genes.Molecular and cellular biology · 2009Article
- Expression of bacterial Rho factor in yeast identifies new factors involved in the functional interplay between transcription and mRNP biogenesis.Molecular and cellular biology · 2009Article
- Getting a transcription factor to only one nucleus following mitosis.PLoS biology · 2008Article
- Nuclear export receptor Xpo1/Crm1 is physically and functionally linked to the spindle pole body in budding yeast.Molecular and cellular biology · 2008Article
- The NDR/LATS family kinase Cbk1 directly controls transcriptional asymmetry.PLoS biology · 2008Article
- Regulation of the yeast Ace2 transcription factor during the cell cycle.The Journal of biological chemistry · 2008Article
- Analysis of P-body assembly in Saccharomyces cerevisiae.Molecular biology of the cell · 2007Article
- Nucleocytoplasmic trafficking is required for functioning of the adaptor protein Sla1p in endocytosis.Traffic (Copenhagen, Denmark) · 2007Article
- Yeast poly(A)-binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export.RNA (New York, N.Y.) · 2005Article
- The small-subunit processome is a ribosome assembly intermediate.Eukaryotic cell · 2004Article
- The Saccharomyces cerevisiae Mob2p-Cbk1p kinase complex promotes polarized growth and acts with the mitotic exit network to facilitate daughter cell-specific localization of Ace2p transcription factor.The Journal of cell biology · 2002Article
- Importin-beta-like nuclear transport receptors.Genome biology · 2001Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Nuclear export of proteins containing leucine-rich nuclear export signals (NESs) is mediated by the NES receptor CRM1/Crm1p. We have carried out a yeast two-hybrid screen with Crm1p as a bait. The Crm1p-interacting clones were subscreened for nuclear export activity in a visual assay utilizing the Crm1p-inhibitor leptomycin B (LMB). This approach identified three Saccharomyces cerevisiae proteins not previously known to have nuclear export activity. These proteins are the 5' RNA triphosphatase Ctl1p, the cell cycle-regulated transcription factor Ace2p, and a protein encoded by the previously uncharacterized open reading frame YDR499W. Mutagenesis analysis show that YDR499Wp contains an NES that conforms to the consensus sequence for leucine-rich NESs. Mutagenesis of Ctl1p and Ace2p were unable to identify specific NES residues. However, a 29-amino-acid region of Ace2p, rich in hydrophobic residues, contains nuclear export activity. Ace2p accumulates in the nucleus at the end of mitosis and activates early-G(1)-specific genes. We now provide evidence that Ace2p is nuclear not only in late M-early G(1) but also during other stages of the cell cycle. This feature of Ace2p localization explains its ability to activate genes such as CUP1, which are not expressed in a cell cycle-dependent manner.
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