ArticleMolecular and cellular biology2000
Evidence for splice site pairing via intron definition in Schizosaccharomyces pombe.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 81 citations in OpenAlex.
- Omics-based dissection of alternative splicing and the regulatory architecture in fungal genomes.Current research in microbial sciences · 2026Review
- Propagation of [D1,2]-type spliceosomal twin introns (stwintrons) inMicrobiology spectrum · 2025Article
- Unveiling a Microexon Switch: Novel Regulation of the Activities of Sugar Assimilation and Plant-Cell-Wall-Degrading Xylanases and Cellulases by Xlr2 inInternational journal of molecular sciences · 2024Article
- Introns: the "dark matter" of the eukaryotic genome.Frontiers in genetics · 2023Review
- Detecting circRNA in purified spliceosomal P complex.Methods (San Diego, Calif.) · 2021Article
- Internally Symmetrical Stwintrons and Related Canonical Introns in Hypoxylaceae Species.Journal of fungi (Basel, Switzerland) · 2021Article
- Transposon clusters as substrates for aberrant splice-site activation.RNA biology · 2021Article
- The changing paradigm of intron retention: regulation, ramifications and recipes.Nucleic acids research · 2019Review
- A spliceosomal twin intron (stwintron) participates in both exon skipping and evolutionary exon loss.Scientific reports · 2019Article
- A novel role of U1 snRNP: Splice site selection from a distance.Biochimica et biophysica acta. Gene regulatory mechanisms · 2019Review
- Intron specificity in pre-mRNA splicing.Current genetics · 2018Review
- Long-read sequencing of nascent RNA reveals coupling among RNA processing events.Genome research · 2018Article
- Characterization of3 Biotech · 2018Article
- Analysis of Competing HIV-1 Splice Donor Sites Uncovers a Tight Cluster of Splicing Regulatory Elements within Exon 2/2b.Journal of virology · 2017Article
- The power of fission: yeast as a tool for understanding complex splicing.Current genetics · 2017Review
- Article
- The Fission Yeast Pre-mRNA-processing Factor 18 (prp18+) Has Intron-specific Splicing Functions with Links to G1-S Cell Cycle Progression.The Journal of biological chemistry · 2016Article
- Selection on Position of Nonsense Codons in Introns.Genetics · 2016Article
- Widespread alternative and aberrant splicing revealed by lariat sequencing.Nucleic acids research · 2015Article
- Regulation of splicing factors by alternative splicing and NMD is conserved between kingdoms yet evolutionarily flexible.Molecular biology and evolution · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Schizosaccharomyces pombe pre-mRNAs are generally multi-intronic and share certain features with pre-mRNAs from Drosophila melanogaster, in which initial splice site pairing can occur via either exon or intron definition. Here, we present three lines of evidence suggesting that, despite these similarities, fission yeast splicing is most likely restricted to intron definition. First, mutating either or both splice sites flanking an internal exon in the S. pombe cdc2 gene produced almost exclusively intron retention, in contrast to the exon skipping observed in vertebrates. Second, we were unable to induce skipping of the internal microexon in fission yeast cgs2, whereas the default splicing pathway excludes extremely small exons in mammals. Because nearly quantitative removal of the downstream intron in cgs2 could be achieved by expanding the microexon, we propose that its retention is due to steric occlusion. Third, several cryptic 5' junctions in the second intron of fission yeast cdc2 are located within the intron, in contrast to their generally exonic locations in metazoa. The effects of expanding and contracting this intron are as predicted by intron definition; in fact, even highly deviant 5' junctions can compete effectively with the standard 5' splice site if they are closer to the 3' splicing signals. Taken together, our data suggest that pairing of splice sites in S. pombe most likely occurs exclusively across introns in a manner that favors excision of the smallest segment possible.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.