ArticleMolecular and cellular biology2000
Schizosaccharomyces pombe Hsk1p is a potential cds1p target required for genome integrity.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 81 citations in OpenAlex.
- Exploring the multifaceted Dbf4-dependent kinase from temporal, spatial, and substrate repertoire perspectives.Communications biology · 2026Review
- Pathways of rDNA copy number homeostasis in Schizosaccharomyces pombe.G3 (Bethesda, Md.) · 2026Article
- Article
- Checkpoint Regulation of Nuclear Tos4 Defines S Phase Arrest in Fission Yeast.G3 (Bethesda, Md.) · 2020Article
- Overlapping Roles in Chromosome Segregation for Heterochromatin Protein 1 (Swi6) and DDK inGenetics · 2019Article
- DDK Has a Primary Role in Processing Stalled Replication Forks to Initiate Downstream Checkpoint Signaling.Neoplasia (New York, N.Y.) · 2018Article
- Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.G3 (Bethesda, Md.) · 2016Article
- Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena.PLoS genetics · 2015Article
- Article
- Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.Molecular and cellular biology · 2012Article
- Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.The Journal of biological chemistry · 2012Article
- Rif1 is a global regulator of timing of replication origin firing in fission yeast.Genes & development · 2012Article
- Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.The Journal of cell biology · 2011Article
- Phosphorylation of Mcm2 modulates Mcm2-7 activity and affects the cell's response to DNA damage.Nucleic acids research · 2011Article
- Article
- Hsk1- and SCF(Pof3)-dependent proteolysis of S. pombe Ams2 ensures histone homeostasis and centromere function.Developmental cell · 2010Article
- Interactions between Swi1-Swi3, Mrc1 and S phase kinase, Hsk1 may regulate cellular responses to stalled replication forks in fission yeast.Genes to cells : devoted to molecular & cellular mechanisms · 2009Article
- Drug design with Cdc7 kinase: a potential novel cancer therapy target.Drug design, development and therapy · 2009Article
- Checkpoint-dependent regulation of origin firing and replication fork movement in response to DNA damage in fission yeast.Molecular and cellular biology · 2009Article
- The Hsk1(Cdc7) replication kinase regulates origin efficiency.Molecular biology of the cell · 2008Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fission yeast Hsk1p kinase is an essential activator of DNA replication. Here we report the isolation and characterization of a novel mutant allele of the gene. Consistent with its role in the initiation of DNA synthesis, hsk1(ts) genetically interacts with several S-phase mutants. At the restrictive temperature, hsk1(ts) cells suffer abnormal S phase and loss of nuclear integrity and are sensitive to both DNA-damaging agents and replication arrest. Interestingly, hsk1(ts) mutants released to the restrictive temperature after early S-phase arrest in hydroxyurea (HU) are able to complete bulk DNA synthesis but they nevertheless undergo an abnormal mitosis. These findings indicate a second role for hsk1 subsequent to HU arrest. Consistent with a later S-phase role, hsk1(ts) is synthetically lethal with Deltarqh1 (RecQ helicase) or rad21ts (cohesin) mutants and suppressed by Deltacds1 (RAD53 kinase) mutants. We demonstrate that Hsk1p undergoes Cds1p-dependent phosphorylation in response to HU and that it is a direct substrate of purified Cds1p kinase in vitro. These results indicate that the Hsk1p kinase is a potential target of Cds1p regulation and that its activity is required after replication initiation for normal mitosis.
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