ArticleMolecular and cellular biology2000
The nicking step in V(D)J recombination is independent of synapsis: implications for the immune repertoire.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 66 citations in OpenAlex.
- Biochemical Characterization of AP Lyase and mBiochemistry · 2017Article
- RAG1/2 induces genomic insertions by mobilizing DNA into RAG1/2-independent breaks.The Journal of experimental medicine · 2017Article
- Regulated complex assembly safeguards the fidelity of Sleeping Beauty transposition.Nucleic acids research · 2017Article
- An autoregulatory mechanism imposes allosteric control on the V(D)J recombinase by histone H3 methylation.Cell reports · 2015Article
- Synapsis alters RAG-mediated nicking at Tcrb recombination signal sequences: implications for the “beyond 12/23” rule.Molecular and cellular biology · 2014Article
- Crosstalk between transposase subunits during cleavage of the mariner transposon.Nucleic acids research · 2014Article
- Modeling of the RAG reaction mechanism.Cell reports · 2014Article
- The emerging diversity of transpososome architectures.Quarterly reviews of biophysics · 2012Review
- Mechanistic basis for RAG discrimination between recombination sites and the off-target sites of human lymphomas.Molecular and cellular biology · 2012Article
- Preventing broken Borrelia telomeres: ResT couples dual hairpin telomere formation with product release.The Journal of biological chemistry · 2010Article
- Review
- Base flipping in V(D)J recombination: insights into the mechanism of hairpin formation, the 12/23 rule, and the coordination of double-strand breaks.Molecular and cellular biology · 2009Article
- A zinc site in the C-terminal domain of RAG1 is essential for DNA cleavage activity.Journal of molecular biology · 2009Article
- H3K4me3 stimulates the V(D)J RAG complex for both nicking and hairpinning in trans in addition to tethering in cis: implications for translocations.Molecular cell · 2009Article
- Structure of the RAG1 nonamer binding domain with DNA reveals a dimer that mediates DNA synapsis.Nature structural & molecular biology · 2009Article
- A non-sequence-specific DNA binding mode of RAG1 is inhibited by RAG2.Journal of molecular biology · 2009Article
- Quantitative analyses of RAG-RSS interactions and conformations revealed by atomic force microscopy.Biochemistry · 2008Article
- Thermal dependency of RAG1 self-association properties.BMC biochemistry · 2008Article
- The beyond 12/23 restriction is imposed at the nicking and pairing steps of DNA cleavage during V(D)J recombination.Molecular and cellular biology · 2007Article
- The structure-specific nicking of small heteroduplexes by the RAG complex: implications for lymphoid chromosomal translocations.DNA repair · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In all of the transposition reactions that have been characterized thus far, synapsis of two transposon ends is required before any catalytic steps (strand nicking or strand transfer) occur. In V(D)J recombination, there have been inconclusive data concerning the role of synapsis in nicking. Synapsis between two 12-substrates or between two 23-substrates has not been ruled out in any studies thus far. Here we provide the first direct tests of this issue. We find that immobilization of signals does not affect their nicking, even though hairpinning is affected in a manner reflecting its known synaptic requirement. We also find that nicking is kinetically a unireactant enzyme-catalyzed reaction. Time courses are no different between nicking seen for a 12-substrate alone and a reaction involving both a 12- and a 23-substrate. Hence, synapsis is neither a requirement nor an effector of the rate of nicking. These results establish V(D)J recombination as the first example of a DNA transposition-type reaction in which catalytic steps begin prior to synapsis, and the results have direct implications for the order of the steps in V(D)J recombination, for the contribution of V(D)J recombination nicks to genomic instability, and for the diversification of the immune repertoire.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.