ArticleMolecular and cellular biology2000
Down-regulation of cyclin D1 expression by prostaglandin A(2) is mediated by enhanced cyclin D1 mRNA turnover.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 134 citations in OpenAlex.
- RNA-Binding RING E3-Ligase DZIP3/hRUL138 Stabilizes Cyclin D1 to Drive Cell-Cycle and Cancer Progression.Cancer research · 2021Article
- The overexpression of AUF1 in colorectal cancer predicts a poor prognosis and promotes cancer progression by activating ERK and AKT pathways.Cancer medicine · 2020Article
- Review
- The mRNA-Binding Protein HuR Is a Kinetically-Privileged Electrophile Sensor.Helvetica chimica acta · 2020Article
- Post-transcriptional regulation of Nrf2-mRNA by the mRNA-binding proteins HuR and AUF1.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- MicroRNA-146b Overexpression Promotes Human Bladder Cancer Invasion via Enhancing ETS2-Mediated mmp2 mRNA Transcription.Molecular therapy. Nucleic acids · 2019Article
- WT1-associated protein is a novel prognostic factor in pancreatic ductal adenocarcinoma.Oncology letters · 2017Article
- Review
- PAR-CLIP analysis uncovers AUF1 impact on target RNA fate and genome integrity.Nature communications · 2014Article
- A novel posttranscriptional mechanism for dietary cholesterol-mediated suppression of liver LDL receptor expression.Journal of lipid research · 2014Article
- Reduction of AUF1-mediated follistatin mRNA decay during glucose starvation protects cells from apoptosis.Nucleic acids research · 2014Article
- Cellular uptake and antiproliferative effects of 11-oxo-eicosatetraenoic acid.Journal of lipid research · 2013Article
- Cellular mRNA decay protein AUF1 negatively regulates enterovirus and human rhinovirus infections.Journal of virology · 2013Article
- Assembly of functional ribonucleoprotein complexes by AU-rich element RNA-binding protein 1 (AUF1) requires base-dependent and -independent RNA contacts.The Journal of biological chemistry · 2013Article
- RNA-binding protein AUF1 represses Dicer expression.Nucleic acids research · 2012Article
- Inhibitory effect of bufalin and cinobufagin on steroidogenesis via the activation of ERK in human adrenocortical cells.British journal of pharmacology · 2012Article
- AUF1 and HuR: possible implications of mRNA stability in thyroid function and disorders.Thyroid research · 2011Article
- Coordinate regulation of GATA-3 and Th2 cytokine gene expression by the RNA-binding protein HuR.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Modulation of neoplastic gene regulatory pathways by the RNA-binding factor AUF1.Frontiers in bioscience (Landmark edition) · 2011Review
- Alternatively expressed domains of AU-rich element RNA-binding protein 1 (AUF1) regulate RNA-binding affinity, RNA-induced protein oligomerization, and the local conformation of bound RNA ligands.The Journal of biological chemistry · 2010Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Prostaglandin A(2) (PGA(2)), an experimental chemotherapeutic agent, causes growth arrest associated with decreased cyclin D1 expression in several cancer cell lines. Here, using human non-small-cell lung carcinoma H1299 cells, we investigated the mechanisms whereby PGA(2) down-regulates cyclin D1 expression. Transcription rates of the cyclin D1 gene, studied using a cyclin D1 promoter-luciferase construct and nuclear run-on assays, were not affected by PGA(2) treatment. Instead, the cyclin D1 mRNA was rendered unstable after exposure to PGA(2). Since the stability of labile mRNA is modulated through binding of proteins to specific mRNA sequences, we sought to identify protein(s) recognizing the cyclin D1 mRNA. In electrophoretic mobility-shift assays using radiolabeled RNA probes derived from different regions of cyclin D1 mRNA, we observed that (i) lysates prepared from PGA(2)-treated cells exhibited enhanced protein-cyclin D1 RNA complex formation; (ii) the kinetics of complex formation correlated closely with that of cyclin D1 mRNA loss; and (iii) binding occurred within a 390-base cyclin D1 3' untranslated region (UTR) (K12). This binding activity could be cross-linked, revealing proteins ranging from 30 to 47 kDa. The RNA-binding protein AUF1, previously associated with the degradation of target mRNAs, bound cyclin D1 mRNA, because anti-AUF1 antibodies were capable of supershifting or immunoprecipitating cyclin D1 mRNA-protein complexes. Finally, insertion of K12 in the 3'UTR of reporter genes markedly reduced the expression and half-life of the resulting chimeric mRNAs in transfected, PGA(2)-treated cells. Our data demonstrate that PGA(2) down-regulates cyclin D1 expression by decreasing cyclin D1 mRNA stability and implicates a 390-base element in the 3'UTR in this regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.