ArticleNucleic acids research2000
Rapid determination of short DNA sequences by the use of MALDI-MS.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- One-pot multi-substrate screening of ligation reactions using PNA tags.Chemical science · 2026Article
- Heterobifunctional modification of DNA for conjugation to solid surfaces.Analytical and bioanalytical chemistry · 2010Article
- Characterization of synthetic oligonucleotides containing biologically important modified bases by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Analytical biochemistry · 2008Article
- A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Nucleic acids research · 2006Article
- MALDI-TOF mass spectrometry: a versatile tool for high-performance DNA analysis.Molecular biotechnology · 2004Review
- RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis.Nucleic acids research · 2003Article
- Simultaneous determination of different DNA sequences by mass spectrometric evaluation of Sanger sequencing reactions.Nucleic acids research · 2002Article
- Base-specific fragmentation of amplified 16S rRNA genes analyzed by mass spectrometry: a tool for rapid bacterial identification.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- DNA sequencing using biotinylated dideoxynucleotides and mass spectrometry.Nucleic acids research · 2001Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a protocol for rapid sequencing of short DNA stretches (15-20 nt) using MALDI-TOF-MS. The protocol is based on the Sanger concept with the modification that double-stranded template DNA is used and all four sequencing reactions are performed in one reaction vial. The sequencing products are separated and detected by MALDI-TOF-MS and the sequence is determined by comparing measured molecular mass differences to expected values. The protocol is optimized for low costs and broad applicability. One reaction typically includes 300 fmol template, 10 pmol primer and 200 pmol each nucleotide monomer. Neither the primer nor any of the nucleotide monomers are labeled. Solid phase purification, concentration and mass spectrometric sample preparation of the sequencing products are accomplished in a few minutes and parallel processing of 96 samples is possible. The mass spectrometric analyses and subsequent sequence read-out require only a few seconds per template.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.