ArticleNucleic acids research2000
Amplification of IgG VH and VL (Fab) from single human plasma cells and B cells.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- B cells, antibody-secreting cells, and virus-specific antibodies respond to herpes simplex virus 2 reactivation in skin.The Journal of clinical investigation · 2021Trial
- Cloning and expression of natively paired antibody fragments from single B cells of immunized rhesus macaques.Frontiers in immunology · 2026Article
- Technologies for Monoclonal Antibody Discovery and Development.International journal of molecular sciences · 2025Review
- Monoclonal antibodies: From magic bullet to precision weapon.Molecular biomedicine · 2024Review
- Resistance of SARS-CoV-2 variants to neutralization by antibodies induced in convalescent patients with COVID-19.Cell reports · 2021Article
- Development of Therapeutic Antibodies and Modulating the Characteristics of Therapeutic Antibodies to Maximize the Therapeutic Efficacy.Biotechnology and bioprocess engineering : BBE · 2021Review
- Development of therapeutic antibodies for the treatment of diseases.Journal of biomedical science · 2020Review
- RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells.PloS one · 2020Article
- Characterization of Individual Human Antibodies That Bind Pertussis Toxin Stimulated by Acellular Immunization.Infection and immunity · 2018Article
- A human monoclonal IgG that binds aβ assemblies and diverse amyloids exhibits anti-amyloid activities in vitro and in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Prognostic B-cell signatures using mRNA-seq in patients with subtype-specific breast and ovarian cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2014Article
- Rapid production of antigen-specific monoclonal antibodies from a variety of animals.BMC biology · 2012Article
- Single B cell antibody technologies.New biotechnology · 2011Review
- Target-selective joint polymerase chain reaction: a robust and rapid method for high-throughput production of recombinant monoclonal antibodies from single cells.BMC biotechnology · 2011Article
- Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cells.BMC biotechnology · 2011Article
- Neutralization of botulinum neurotoxin by a human monoclonal antibody specific for the catalytic light chain.PloS one · 2008Article
- In situ isolation of immunoglobulin sequences expressed by single tumor-infiltrating B cells using laser-assisted microdissection.Molecular biotechnology · 2005Article
- Recurrent variable region gene usage and somatic mutation in the human antibody response to the capsular polysaccharide of Streptococcus pneumoniae type 23F.Infection and immunity · 2002Article
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amplification of human immunoglobulin has many potential applications such as analysis of clonality, isolation of immunogenic antigens and antigen-specific immunotherapy. Here we describe a method for amplification of human immunoglobulin heavy and light chains from single B lymphocytes or plasma cells. Cells are isolated by FACS, and Ig is amplified by semi-nested RT-PCR. The method is versatile, sensitive and reliable: it provides appropriately paired heavy and light chains, requiring as little as 2 days to produce amplified Fab DNA from human tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.