ArticleNucleic acids research2000
Expansion of the (CTG)(n) repeat in the 5'-UTR of a reporter gene impedes translation.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Zfp106 binds to G-quadruplex RNAs and inhibits RAN translation and formation of RNA foci caused by G4C2 repeats.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- RNA biology of disease-associated microsatellite repeat expansions.Acta neuropathologica communications · 2017Review
- Trojan triplets: RNA-based pathomechanisms for muscle dysfunction in Huntington's disease.The Journal of general physiology · 2017Article
- The role of break-induced replication in large-scale expansions of (CAG)Nature structural & molecular biology · 2017Article
- RNA toxicity induced by expanded CAG repeats in Huntington's disease.Brain pathology (Zurich, Switzerland) · 2016Review
- Computational investigation of RNA CUG repeats responsible for myotonic dystrophy 1.Journal of chemical theory and computation · 2015Article
- Patterns of microsatellite evolution inferred from the Helianthus annuus (Asteraceae) transcriptome.Journal of genetics · 2014Article
- Length-dependent CTG·CAG triplet-repeat expansion in myotonic dystrophy patient-derived induced pluripotent stem cells.Human molecular genetics · 2013Article
- Triplet repeat RNA structure and its role as pathogenic agent and therapeutic target.Nucleic acids research · 2012Review
- Development of SSR and gene-targeted markers for construction of a framework linkage map of Catharanthus roseus.Annals of botany · 2011Article
- A GAG trinucleotide-repeat polymorphism in the gene for glutathione biosynthetic enzyme, GCLC, affects gene expression through translation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Repeat associated non-ATG translation initiation: one DNA, two transcripts, seven reading frames, potentially nine toxic entities!PLoS genetics · 2011Review
- Trinucleotide repeats in human genome and exome.Nucleic acids research · 2010Article
- Structural insights into CUG repeats containing the 'stretched U-U wobble': implications for myotonic dystrophy.Nucleic acids research · 2009Article
- Functional significance of the GAG trinucleotide-repeat polymorphism in the gene for the catalytic subunit of gamma-glutamylcysteine ligase.Free radical biology & medicine · 2008Article
- The biological effects of simple tandem repeats: lessons from the repeat expansion diseases.Genome research · 2008Review
- Effects of Friedreich's ataxia (GAA)n*(TTC)n repeats on RNA synthesis and stability.Nucleic acids research · 2007Article
- Structures of trinucleotide repeats in human transcripts and their functional implications.Nucleic acids research · 2003Review
- Anticipation and CAG*CTG repeat expansion in schizophrenia and bipolar affective disorder.Current psychiatry reports · 2003Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Effects of d(CAG)(n).d(CTG)(n) repeats on expression of a reporter gene in human cell culture were studied using transient transfection, RNase protection and coupled transcription/translation assays. Cloning these repeats into the reporter 3'-UTR did not affect gene functioning. In contrast, placing the repeats in the reporter 5'-UTR led to strong inhibition of expression. This inhibition depended on the repeat orientation, being prominent only when the (CTG)(n) tracts were in the sense strand for transcription. Further, the strength of inhibition increased exponentially with an increase in repeat length. Our data indicate that expanded (CTG)(n) repeats prevent efficient translation of the reporter mRNA both in vitro and in vivo. We suggest that formation of stable hairpins by (CUG)(n) runs of increasing length in the 5'-UTR of a mRNA progressively inhibits the scanning step of translation initiation. This points to a novel mechanism of regulating gene expression by expandable d(CTG)(n) repeats.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.