ArticleNucleic acids research2000
Transcription efficiency of human polymerase III genes in vitro does not depend on the RNP-forming autoantigen La.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- AbioRxiv : the preprint server for biology · 2024Article
- The La protein counteracts cisplatin-induced cell death by stimulating protein synthesis of anti-apoptotic factor Bcl2.Oncotarget · 2016Article
- Combinations of siRNAs against La Autoantigen with NS5B or hVAP-A Have Additive Effect on Inhibition of HCV Replication.Hepatitis research and treatment · 2016Article
- SUMO-Modification of the La Protein Facilitates Binding to mRNA In Vitro and in Cells.PloS one · 2016Article
- Cell growth- and differentiation-dependent regulation of RNA polymerase III transcription.Cell cycle (Georgetown, Tex.) · 2010Review
- Transcription termination by nuclear RNA polymerases.Genes & development · 2009Article
- Visual analysis of the yeast 5S rRNA gene transcriptome: regulation and role of La protein.Molecular and cellular biology · 2008Article
- Transcriptional regulation of human small nuclear RNA genes.Biochimica et biophysica acta · 2008Review
- Human La is found at RNA polymerase III-transcribed genes in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Assembly and isolation of intermediate steps of transcription complexes formed on the human 5S rRNA gene.Nucleic acids research · 2003Article
- Methylphosphate cap structure in small RNAs reduces the affinity of RNAs to La protein.Gene expression · 2002Article
- La protein and its associated small nuclear and nucleolar precursor RNAs.Gene expression · 2002Review
- Phosphorylation of the Saccharomyces cerevisiae La protein does not appear to be required for its functions in tRNA maturation and nascent RNA stabilization.RNA (New York, N.Y.) · 2001Article
- Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human.Nucleic acids research · 2001Review
- Translational Control of the Picornavirus Phenotype.Molecular biology · 2001Article
- Recognition of nascent RNA by the human La antigen: conserved and divergent features of structure and function.Molecular and cellular biology · 2001Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription of class III genes is conducted by multi-protein complexes consisting of polymerase III itself and several transcription factors. We established a reconstituted in vitro transcription system from which the autoantigen La was removed by immunodepletion. This system showed no RNP formation, but was still fully active in transcription. Supplementing such La-free transcription reactions with recombinant La restored the formation of La complexes with the newly synthesised RNA, but did not lead to enhanced transcription efficiency. Furthermore, we developed a technique for the generation and isolation of transcription complexes, assembled from purified transcription factors and isolated by glycerol centrifugation. These complexes were fully competent to re-initiate RNA synthesis but they were not associated with La and their transcription rate could not be stimulated by addition of recombinant La. Therefore, we conclude that La does not act as a human polymerase III transcription factor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.