ArticleNucleic acids research2000
Characterization of the cold stress-induced cyanobacterial DEAD-box protein CrhC as an RNA helicase.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 67 citations in OpenAlex.
- Liquid-Liquid Phase Separation of the DEAD-Box Cyanobacterial RNA Helicase Redox (CrhR) into Dynamic Membraneless Organelles inApplied and environmental microbiology · 2023Article
- AGC/AKT Protein Kinase SCH9 Is Critical to Pathogenic Development and Overwintering Survival inJournal of fungi (Basel, Switzerland) · 2022Article
- Review
- TheRNA (New York, N.Y.) · 2020Article
- Both Enolase and the DEAD-Box RNA Helicase CrhB Can Form Complexes with RNase E inApplied and environmental microbiology · 2020Article
- Multifunctional RNA Binding Protein OsTudor-SN in Storage Protein mRNA Transport and Localization.Plant physiology · 2017Article
- Large scale phosphoprotein profiling to explore Drosophila cold acclimation regulatory mechanisms.Scientific reports · 2017Article
- Physiological Mechanisms Only Tell Half Story: Multiple Biological Processes are involved in Regulating Freezing Tolerance of Imbibed Lactuca sativa Seeds.Scientific reports · 2017Article
- Cyanobacterial RNA Helicase CrhR Localizes to the Thylakoid Membrane Region and Cosediments with Degradosome and Polysome Complexes in Synechocystis sp. Strain PCC 6803.Journal of bacteriology · 2016Article
- Effects of low temperature on tropical and temperate isolates of marine Synechococcus.The ISME journal · 2016Article
- Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment.Frontiers in microbiology · 2015Article
- AtRH57, a DEAD-box RNA helicase, is involved in feedback inhibition of glucose-mediated abscisic acid accumulation during seedling development and additively affects pre-ribosomal RNA processing with high glucose.The Plant journal : for cell and molecular biology · 2014Article
- Conformational transitions in human translin enable nucleic acid binding.Nucleic acids research · 2013Article
- RNA helicases: diverse roles in prokaryotic response to abiotic stress.RNA biology · 2013Review
- Autoregulation of RNA helicase expression in response to temperature stress in Synechocystis sp. PCC 6803.PloS one · 2012Article
- Article
- Engineering cold stress tolerance in crop plants.Current genomics · 2011Article
- Allosteric activation of the ATPase activity of the Escherichia coli RhlB RNA helicase.The Journal of biological chemistry · 2008Article
- Dead-box proteins: a family affair--active and passive players in RNP-remodeling.Nucleic acids research · 2006Review
- RNA helicases and abiotic stress.Nucleic acids research · 2006Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have shown previously that CrhC is a unique member of the DEAD-box family of RNA helicases whose expression occurs specifically under conditions of cold stress. Here we show that recombinant His-tagged CrhC, purified from Escherichia coli, is an ATP-independent RNA binding protein possessing RNA-dependent ATPase activity which is stimulated most efficiently by rRNA and polysome preparations. RNA strand displacement assays indicate that CrhC possesses RNA unwinding activity that is adenosine nucleotide specific. Unwinding of partially duplexed RNA proceeds in the 5'-->3' but not the 3'-->5' direction using standard assay conditions. Immunoprecipitation and far-western analysis indicate that CrhC is a component of a multisubunit complex, interacting specifically with a 37 kDa polypeptide. We propose that CrhC unwinds cold-stabilized secondary structure in the 5'-UTR of RNA during cold stress.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.