ArticleJournal of virology2000
Activation of transcription of the human cytomegalovirus early UL4 promoter by the Ets transcription factor binding element.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- NovelInternational journal of molecular sciences · 2021Article
- Comparative Genomics of Herpesviridae Family to Look for Potential Signatures of Human Infecting Strains.International journal of genomics · 2016Article
- New cell-signaling pathways for controlling cytomegalovirus replication.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2014Review
- Human cytomegalovirus transcriptome activity differs during replication in human fibroblast, epithelial and astrocyte cell lines.The Journal of general virology · 2012Article
- The presence of p53 influences the expression of multiple human cytomegalovirus genes at early times postinfection.Journal of virology · 2009Article
- The autoregulatory and transactivating functions of the human cytomegalovirus IE86 protein use independent mechanisms for promoter binding.Journal of virology · 2007Article
- Localization of ERK/MAP kinase is regulated by the alphaherpesvirus tegument protein Us2.Journal of virology · 2006Article
- Role of regulatory elements and the MAPK/ERK or p38 MAPK pathways for activation of human cytomegalovirus gene expression.Journal of virology · 2002Article
- Identification of glycoprotein gpTRL10 as a structural component of human cytomegalovirus.Journal of virology · 2002Article
- Characterization of a human cytomegalovirus with phosphorylation site mutations in the immediate-early 2 protein.Journal of virology · 2002Article
- Cyclophilin A as a target in the treatment of cytomegalovirus infections.Antiviral chemistry & chemotherapyReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The human cytomegalovirus (HCMV) early UL4 promoter has served as a useful model for studying the activation of early viral gene expression. Previous transient-transfection experiments detected cis-acting elements (the NF-Y site and site 2) upstream of the transcriptional start site (L. Huang and M. F. Stinski, J. Virol. 69:7612-7621, 1995). The roles of two of these sites, the NF-Y site and site 2, in the context of the viral genome were investigated further by comparing mRNA levels from the early UL4 promoter in human foreskin fibroblasts infected by recombinant viruses with either wild-type or mutant cis-acting elements. Steady-state mRNA levels from the UL4 promoter with a mutation in the NF-Y site were comparable to that of wild type. A mutation in an Elk-1 site plus putative IE86 protein binding sites decreased the steady-state mRNA levels compared to the wild type at early times after infection. Electrophoretic mobility shift assays and antibody supershifts detected the binding of cellular transcription factor Elk-1 to site 2 DNA with infected nuclear extracts but not with mock-infected nuclear extracts. The role of cellular transcription factors activated by the mitogen activated protein kinase/extracellular signal-regulated kinase pathway in activating transcription from early viral promoters is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.