ArticleBiophysical journal2000
Tracking single proteins within cells.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 268 citations in OpenAlex.
- Article
- Single-molecule localization and diffusivity microscopy reveals dynamic biomolecular organization in living cells.Nature methods · 2026Article
- Exploring Biophysics at the Membrane with Single-Molecule TIRF Microscopy.Advances in experimental medicine and biology · 2026Review
- High-Speed Interferometric Scattering Tracking Microscopy of Compartmentalized Lipid Diffusion in Living Cells.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
- Single molecule dynamics in a virtual cell combining a 3-dimensional matrix model with random walks.Scientific reports · 2024Article
- Particle Localization Using Local Gradients and Its Application to Nanometer Stabilization of a Microscope.ACS nano · 2022Article
- Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model.Nature communications · 2022Article
- Article
- Probing local chromatin dynamics by tracking telomeres.Biophysical journal · 2022Article
- A Protocol for Studying Transcription Factor Dynamics Using Fast Single-Particle Tracking and Spot-On Model-Based Analysis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- TRAIT2D: a Software for Quantitative Analysis of Single Particle Diffusion Data.F1000Research · 2021Article
- Extracting Transition Rates in Particle Tracking Using Analytical Diffusion Distribution Analysis.Biophysical journal · 2020Article
- 3D tracking of extracellular vesicles by holographic fluorescence imaging.Science advances · 2020Article
- Auxin-induced signaling protein nanoclustering contributes to cell polarity formation.Nature communications · 2020Article
- Secretion of Phospholipase Dδ Functions as a Regulatory Mechanism in Plant Innate Immunity.The Plant cell · 2019Article
- Septins organize endoplasmic reticulum-plasma membrane junctions for STIM1-ORAI1 calcium signalling.Scientific reports · 2019Article
- Protein motion in the nucleus: from anomalous diffusion to weak interactions.Biochemical Society transactions · 2018Review
- SPT and Imaging FCS Provide Complementary Information on the Dynamics of Plasma Membrane Molecules.Biophysical journal · 2018Article
- Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells.BMC plant biology · 2018Article
- Article
9 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present experiments in which single proteins were imaged and tracked within mammalian cells. Single proteins of R-phycoerythrin (RPE) were imaged by epifluorescence microscopy in the nucleoplasm and cytoplasm at 71 frames/s. We acquired two-dimensional trajectories of proteins (corresponding to the projection of three-dimensional trajectories onto the plane of focus) for an average of 17 frames in the cytoplasm and 16 frames in the nucleus. Diffusion constants were determined from linear fits to the mean square displacement and from the mean displacement squared per frame. We find that the distribution of diffusion constants for RPE within cells is broader than the distributions obtained from RPE in a glycerol solution, from a Monte Carlo simulation, and from the theoretical distribution for simple diffusion. This suggests that on the time scales of our measurements, the motion of single RPE proteins in the cytoplasm and nucleoplasm cannot be modeled by simple diffusion with a unique diffusion constant. Our results demonstrate that it is possible to follow the motion of single proteins within cells and that the technique of single molecule tracking can be used to probe the dynamics of intracellular macromolecules.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.