ArticleBiophysical journal2000
Characterization of single-cell electroporation by using patch-clamp and fluorescence microscopy.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Microfluidic Electroporation Arrays for Investigating Electroporation-Induced Cellular Rupture Dynamics.Biosensors · 2024Article
- Single-cell individualized electroporation with real-time impedance monitoring using a microelectrode array chip.Microsystems & nanoengineering · 2020Article
- Current Trends of Microfluidic Single-Cell Technologies.International journal of molecular sciences · 2018Review
- Characterization of Cell Membrane Permeability In Vitro Part I: Transport Behavior Induced by Single-Pulse Electric Fields.Technology in cancer research & treatment · 2018Article
- Characterization of Cell Membrane Permeability In Vitro Part II: Computational Model of Electroporation-Mediated Membrane Transport.Technology in cancer research & treatment · 2018Article
- Magneto-elasto-electroporation (MEEP): In-vitro visualization and numerical characteristics.Scientific reports · 2016Article
- Controllable in-situ cell electroporation with cell positioning and impedance monitoring using micro electrode array.Scientific reports · 2016Article
- Comparison between direct and reverse electroporation of cells in situ: a simulation study.Physiological reports · 2016Article
- Mechanisms of transfer of bioactive molecules through the cell membrane by electroporation.European biophysics journal : EBJ · 2015Review
- Probing enzymatic activity inside single cells.Analytical chemistry · 2013Article
- Single-cell juxtacellular transfection and recording technique.Pflugers Archiv : European journal of physiology · 2013Article
- Nanofountain probe electroporation (NFP-E) of single cells.Nano letters · 2013Article
- Electroporation followed by electrochemical measurement of quantal transmitter release from single cells using a patterned microelectrode.Lab on a chip · 2013Article
- A lipocentric view of peptide-induced pores.European biophysics journal : EBJ · 2011Review
- Distinguishing splanchnic nerve and chromaffin cell stimulation in mouse adrenal slices with fast-scan cyclic voltammetry.American journal of physiology. Cell physiology · 2011Article
- Kinetics of NF-κB nucleocytoplasmic transport probed by single-cell screening without imaging.Lab on a chip · 2010Article
- Microfluidic electroporation of tumor and blood cells: observation of nucleus expansion and implications on selective analysis and purging of circulating tumor cells.Integrative biology : quantitative biosciences from nano to macro · 2010Article
- Control of the release of freely diffusing molecules in single-cell electroporation.Analytical chemistry · 2009Article
- Nanopipette delivery of individual molecules to cellular compartments for single-molecule fluorescence tracking.Biophysical journal · 2007Article
- Calcium indicator loading of neurons using single-cell electroporation.Pflugers Archiv : European journal of physiology · 2007Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electroporation of single NG108-15 cells with carbon-fiber microelectrodes was characterized by patch-clamp recordings and fluorescence microscopy. To minimize adverse capacitive charging effects, the patch-clamp pipette was sealed on the cell at a 90(o) angle with respect to the microelectrodes where the applied potential reaches a minimum. From transmembrane current responses, we determined the electric field strengths necessary for ion-permeable pore formation and investigated the kinetics of pore opening and closing as well as pore open times. From both patch-clamp and fluorescence microscopy experiments, the threshold transmembrane potentials for dielectric breakdown of NG108-15 cells, using 1-ms rectangular waveform pulses, was approximately 250 mV. The electroporation pulse preceded pore formation, and analyte entry into the cells was dictated by concentration, and membrane resting potential driving forces. By stepwise moving a cell out of the focused field while measuring the transmembrane current response during a supramaximal pulse, we show that cells at a distance of approximately 30 microm from the focused field were not permeabilized.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.