ArticleBiophysical journal2000
Concurrent and independent binding of Fcgamma receptors IIa and IIIb to surface-bound IgG.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Biomembrane force probe (BFP): Design, advancements, and recent applications to live-cell mechanobiology.Exploration (Beijing, China) · 2023Review
- Cooperative binding of T cell receptor and CD4 to peptide-MHC enhances antigen sensitivity.Nature communications · 2022Article
- The kinetics of E-selectin- and P-selectin-induced intermediate activation of integrin αLβ2 on neutrophils.Journal of cell science · 2021Article
- PD-1 suppresses TCR-CD8 cooperativity during T-cell antigen recognition.Nature communications · 2021Article
- Plasma Membrane Integrates Biophysical and Biochemical Regulation to Trigger Immune Receptor Functions.Frontiers in immunology · 2021Review
- GPRC5D is a target for the immunotherapy of multiple myeloma with rationally designed CAR T cells.Science translational medicine · 2019Article
- Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment.Nature communications · 2018Article
- Microfluidic auto-alignment of protein patterns for dissecting multi-receptor crosstalk in platelets.Lab on a chip · 2018Article
- Dual Biomembrane Force Probe enables single-cell mechanical analysis of signal crosstalk between multiple molecular species.Scientific reports · 2017Article
- Effects of anchor structure and glycosylation of Fcγ receptor III on ligand binding affinity.Molecular biology of the cell · 2016Article
- Immobilized IL-8 Triggers Phagocytosis and Dynamic Changes in Membrane Microtopology in Human Neutrophils.Annals of biomedical engineering · 2015Article
- Controlled One-on-One Encounters between Immune Cells and Microbes Reveal Mechanisms of Phagocytosis.Biophysical journal · 2015Review
- Designing cell-targeted therapeutic proteins reveals the interplay between domain connectivity and cell binding.Biophysical journal · 2014Article
- Adhesion frequency assay for in situ kinetics analysis of cross-junctional molecular interactions at the cell-cell interface.Journal of visualized experiments : JoVE · 2011Article
- Blurred line between chemotactic chase and phagocytic consumption: an immunophysical single-cell perspective.Journal of cell science · 2011Review
- Target-specific mechanics of phagocytosis: protrusive neutrophil response to zymosan differs from the uptake of antibody-tagged pathogens.Journal of cell science · 2011Article
- Dynamics of the interaction of human IgG subtype immune complexes with cells expressing R and H allelic forms of a low-affinity Fc gamma receptor CD32A.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Active site formation, not bond kinetics, limits adhesion rate between human neutrophils and immobilized vascular cell adhesion molecule 1.Biophysical journal · 2009Article
- Measuring diffusion and binding kinetics by contact area FRAP.Biophysical journal · 2008Article
- Two stage cadherin kinetics require multiple extracellular domains but not the cytoplasmic region.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Fc receptor-antibody interactions are key mechanisms through which antibody effector functions are mediated. Neutrophils coexpress two low-affinity Fcgamma receptors, CD16b (FcgammaRIIIb) and CD32a (FcgammaRIIa), possessing overlapping ligand binding specificities but distinct membrane anchor and signaling capacities. Using K562 cell transfectants as a model, the kinetics of both separate and concurrent binding of CD16b and CD32a to surface-bound IgG ligands were studied. CD16b bound human IgG with 2-3 times higher affinity than did CD32a (A(c)K(a) = 4.1 and 1.6 x 10(-7) microm(4), respectively) and both FcgammaRs had similar reverse kinetic rates (k(r) = 0.5 and 0.4 s(-1), respectively). Because CD16b is expressed on neutrophils at a 4-5 times higher density than CD32a, our results suggest that CD16b plays the dominant role in binding of neutrophils to immobilized IgG. The question of possible cross-regulation of binding affinity between CD16b and CD32a was investigated using our multispecies concurrent binding model (Zhu and Williams, Biophys. J. 79:1850-1857, 2000). Because the model assumes independent binding (no cooperation among different species), the excellent agreement between the model predictions and the experimental data suggests that, when coexpressed on K562 cells, these two FcgammaRs do not interact in a manner that alters the kinetic rates of either molecule.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.