ArticleBiophysical journal2000
Concurrent binding to multiple ligands: kinetic rates of CD16b for membrane-bound IgG1 and IgG2.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Biomembrane force probe (BFP): Design, advancements, and recent applications to live-cell mechanobiology.Exploration (Beijing, China) · 2023Review
- Cooperative binding of T cell receptor and CD4 to peptide-MHC enhances antigen sensitivity.Nature communications · 2022Article
- The kinetics of E-selectin- and P-selectin-induced intermediate activation of integrin αLβ2 on neutrophils.Journal of cell science · 2021Article
- PD-1 suppresses TCR-CD8 cooperativity during T-cell antigen recognition.Nature communications · 2021Article
- The Neutrophil: The Underdog That Packs a Punch in the Fight against Cancer.International journal of molecular sciences · 2020Review
- Dual Biomembrane Force Probe enables single-cell mechanical analysis of signal crosstalk between multiple molecular species.Scientific reports · 2017Article
- Effects of anchor structure and glycosylation of Fcγ receptor III on ligand binding affinity.Molecular biology of the cell · 2016Article
- Allosteric Regulation of E-Cadherin Adhesion.The Journal of biological chemistry · 2015Article
- Melanoma upregulates ICAM-1 expression on endothelial cells through engagement of tumor CD44 with endothelial E-selectin and activation of a PKCα-p38-SP-1 pathway.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Adhesion frequency assay for in situ kinetics analysis of cross-junctional molecular interactions at the cell-cell interface.Journal of visualized experiments : JoVE · 2011Article
- Determining beta2-integrin and intercellular adhesion molecule 1 binding kinetics in tumor cell adhesion to leukocytes and endothelial cells by a gas-driven micropipette assay.The Journal of biological chemistry · 2011Article
- Dynamics of the interaction of human IgG subtype immune complexes with cells expressing R and H allelic forms of a low-affinity Fc gamma receptor CD32A.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Measuring diffusion and binding kinetics by contact area FRAP.Biophysical journal · 2008Article
- Two stage cadherin kinetics require multiple extracellular domains but not the cytoplasmic region.The Journal of biological chemistry · 2008Article
- Probabilistic modeling of rosette formation.Biophysical journal · 2006Article
- Two-dimensional kinetics regulation of alphaLbeta2-ICAM-1 interaction by conformational changes of the alphaL-inserted domain.The Journal of biological chemistry · 2005Article
- Diffusion of microspheres in shear flow near a wall: use to measure binding rates between attached molecules.Biophysical journal · 2001Article
- Concurrent and independent binding of Fcgamma receptors IIa and IIIb to surface-bound IgG.Biophysical journal · 2000Article
- Modeling concurrent binding of multiple molecular species in cell adhesion.Biophysical journal · 2000Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
CD16b (FcgammaRIIIb) is the most common receptor for the Fc domain of IgG on leukocytes. The binding of Fc receptors to immunoglobulin triggers a wide array of immune responses. In published assays measuring the reaction of CD16b with isotypes of soluble IgG, the affinity for IgG1 was low and that for IgG2 was undetectable. Here we report the first measurement of kinetic rates of CD16b binding to membrane-bound IgG isotypes-a physically distinct and physiologically more relevant presentation-using a recently developed micropipette method. In contrast to the soluble data, we found clearly measurable IgG2 binding, with a forward kinetic rate six-fold lower than that of IgG1 but with an equilibrium affinity only threefold lower. This suggests a nonnegligible role for IgG2 in Fc-mediated immune responses, particularly in longer duration contacts. The binding constants were measured from two sets of experiments. Single-isotype experiments were analyzed by an existing model (, Biophys. J. 75:1553-1572). The resulting kinetic rates were used as input to an extended model (, Biophys. J. 79:1850-1857.) to predict the results of mixed-isotype experiments. This design enabled rigorous validation of the concurrent binding model through a test of its predictive ability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.