ArticleBiophysical journal2000
Diffusion in inhomogeneous media: theory and simulations applied to whole cell photobleach recovery.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
65 citing papers in PubMed.
- TUDCA modulates drug bioavailability to regulate resistance to acute ER stress inMolecular biology of the cell · 2025Article
- Beyond analytic solution: Analysis of FRAP experiments by spatial simulation of the forward problem.Biophysical journal · 2023Article
- Using Fluorescence Recovery After Photobleaching data to uncover filament dynamics.PLoS computational biology · 2022Article
- VASP localization to lipid bilayers induces polymerization driven actin bundle formation.Molecular biology of the cell · 2022Article
- Indirect Methods To Measure Unfolded Proteins In Living Cells Using Fluorescent Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Accumulation of Neurofascin at Nodes of Ranvier Is Regulated by a Paranodal Switch.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Size-dependent secretory protein reflux into the cytosol in association with acute endoplasmic reticulum stress.Traffic (Copenhagen, Denmark) · 2020Article
- The Unfolded Protein Response: Detecting and Responding to Fluctuations in the Protein-Folding Capacity of the Endoplasmic Reticulum.Cold Spring Harbor perspectives in biology · 2019Review
- The Development and Enhancement of FRAP as a Key Tool for Investigating Protein Dynamics.Biophysical journal · 2018Review
- Article
- The SLE variant Ala71Thr of BLK severely decreases protein abundance and binding to BANK1 through impairment of the SH3 domain function.Genes and immunity · 2016Article
- Universal Approach to FRAP Analysis of Arbitrary Bleaching Patterns.Scientific reports · 2015Article
- Dissecting protein reaction dynamics in living cells by fluorescence recovery after photobleaching.Nature protocols · 2015Article
- Light and life in Baltimore--and beyond.Biophysical journal · 2015Article
- Engineering and exploitation of a fluorescent HIV-1 gp120 for live cell CD4 binding assays.Virology · 2015Article
- Validation of Normalizations, Scaling, and Photofading Corrections for FRAP Data Analysis.PloS one · 2015Article
- Munc18-1 redistributes in nerve terminals in an activity- and PKC-dependent manner.The Journal of cell biology · 2014Article
- Approaches to imaging unfolded secretory protein stress in living cells.Endoplasmic reticulum stress in diseases · 2014Article
- Parameter importance in FRAP acquisition and analysis: a simulation approach.Biophysical journal · 2013Article
- ERdj3 regulates BiP occupancy in living cells.Journal of cell science · 2013Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
A continuum description for diffusion in a simple model for an inhomogeneous but isotropic media is derived and implemented numerically. The locally averaged density of diffusible marker is input from experiment to define the sample. Then a single additional parameter, the effective diffusion constant, permits the quantitative simulation of diffusive relaxation from any initial condition. Using this simulation, it is possible to model the recovery of a fluorescently tagged protein in the endoplasmic reticulum (ER) after photobleaching a substantial region of a live cell, and fit an effective diffusion constant which is a property both of the geometry of the ER and the marker. Such quantitative measurements permit inferences about the topology and internal organization of this organelle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.