ArticleThe Biochemical journal2000
Identification of a novel 45 kDa protein (JP-45) from rabbit sarcoplasmic-reticulum junctional-face membrane.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Review
- Calreticulin-Enigmatic Discovery.Biomolecules · 2024Review
- Proteomic profiling of impaired excitation-contraction coupling and abnormal calcium handling in muscular dystrophy.Proteomics · 2022Review
- Article
- Case report: NovelFrontiers in oncology · 2022Article
- Improper Remodeling of Organelles Deputed to CaInternational journal of molecular sciences · 2021Review
- Extraocular muscle function is impaired inThe Journal of general physiology · 2019Article
- Calcium Dynamics Mediated by the Endoplasmic/Sarcoplasmic Reticulum and Related Diseases.International journal of molecular sciences · 2017Review
- Role of the JP45-Calsequestrin Complex on Calcium Entry in Slow Twitch Skeletal Muscles.The Journal of biological chemistry · 2016Article
- STIM proteins and the endoplasmic reticulum-plasma membrane junctions.Annual review of biochemistry · 2011Review
- Minor sarcoplasmic reticulum membrane components that modulate excitation-contraction coupling in striated muscles.The Journal of physiology · 2009Review
- Structure of frozen-hydrated triad junctions: a case study in motif searching inside tomograms.Journal of structural biology · 2009Article
- Loss of skeletal muscle strength by ablation of the sarcoplasmic reticulum protein JP45.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Bridging the myoplasmic gap: recent developments in skeletal muscle excitation-contraction coupling.Journal of muscle research and cell motility · 2007Review
- The junctional SR protein JP-45 affects the functional expression of the voltage-dependent Ca2+ channel Cav1.1.Journal of cell science · 2006Article
- A possible role of the junctional face protein JP-45 in modulating Ca2+ release in skeletal muscle.The Journal of physiology · 2006Article
- Calcium signalling in muscle: a milestone for modulation studies.The Journal of physiology · 2006Article
- Novel sarco(endo)plasmic reticulum proteins and calcium homeostasis in striated muscles.Journal of muscle research and cell motility · 2005Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Using a biochemical/immunological approach to analyse the protein constituents of skeletal-muscle junctional-face membrane (JFM), we identified a 45 kDa protein. Its N-terminal amino acid was blocked, but the amino acid sequence obtained from several peptides after proteolytic treatment did not significantly match that of any protein present in the SwissProt and NCBI (National Center for Biotechnology Information) databases. We synthesized a peptide whose sequence matched that of one of the peptides obtained after CNBr cleavage of the 45 kDa protein; the peptide was conjugated to a carrier and used to raise antibodies. The antiserum was used to study in more detail the biochemical characteristics of the novel 45 kDa protein. Analysis of the proteins present in different subcellular membrane fractions show that the novel 45 kDa polypeptide: (i) is an integral membrane constituent present both in neonatal and adult skeletal-muscle sarcoplasmic reticulum; (ii) is selectively localized in the JFM; (iii) is not present in microsomes obtained from rabbit heart, liver or kidney. Immunoprecitation with anti-(45 kDa protein) antibody indicates that the 45 kDa protein is part of a complex which can be phosphorylated in vitro by the catalytic subunit of protein kinase A.
Indexed as
Identifiers
11023841PMC1221391W2057661416What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.