ArticleJournal of clinical microbiology2000
Comparison of six commercial DNA extraction kits for recovery of cytomegalovirus DNA from spiked human specimens.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 75 citations in OpenAlex.
- Endothelial nitric oxide synthase (G894T) gene polymorphism in a random sample of the Egyptian population: comparison with myocardial infarction patients.Genetic testing and molecular biomarkers · 2012Trial
- A critical review of point-of-care diagnostic technologies to combat viral pandemics.Analytica chimica acta · 2021Review
- Increasing Cytomegalovirus Detection Rate from Respiratory Tract Specimens by a New Laboratory-Developed Automated Molecular Diagnostic Test.Microorganisms · 2020Article
- Biases during DNA extraction affect bacterial and archaeal community profile of anaerobic digestion samples.3 Biotech · 2017Article
- Hookworm infection is associated with decreased CD4+ T cell counts in HIV-infected adult Ugandans.PLoS neglected tropical diseases · 2017Article
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- C242T polymorphism of NADPH oxidase p22phox gene reduces the risk of coronary artery disease in a random sample of Egyptian population.Molecular biology reports · 2014Article
- Article
- A novel, multi-parallel, real-time polymerase chain reaction approach for eight gastrointestinal parasites provides improved diagnostic capabilities to resource-limited at-risk populations.The American journal of tropical medicine and hygiene · 2013Article
- DNA extract characterization process for microbial detection methods development and validation.BMC research notes · 2012Article
- Generic microtiter plate assay for triaging clinical specimens prior to genotyping of human papillomavirus DNA via consensus PCR.Journal of clinical microbiology · 2011Article
- Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications.BMC research notes · 2010Article
- Protocol for the quantitative assessment of DNA concentration and damage (fragmentation and nicks).Nature protocols · 2009Article
- Detection of a molecular biomarker for zygomycetes by quantitative PCR assays of plasma, bronchoalveolar lavage, and lung tissue in a rabbit model of experimental pulmonary zygomycosis.Journal of clinical microbiology · 2008Article
- Comparison of polymerase chain reaction methods for the detection of Theileria equi infection using whole blood compared with pre-extracted DNA samples as PCR templates.Tropical animal health and production · 2007Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Comparison of methods for detection of vaccinia virus in patient specimens.Journal of clinical microbiology · 2005Article
- Squirrel monkeys support replication of BK virus more efficiently than simian virus 40: an animal model for human BK virus infection.Journal of virology · 2005Article
- Comparative analysis of the Schleicher and Schuell IsoCode Stix DNA isolation device and the Qiagen QIAamp DNA Mini Kit.Journal of clinical microbiology · 2004Article
- Pre-PCR processing: strategies to generate PCR-compatible samples.Molecular biotechnology · 2004Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We evaluated six commercially available DNA extraction kits for their ability to recover DNA from various dilutions of cytomegalovirus (CMV) added to four different specimens: bronchoalveolar lavage, cerebral spinal fluid, plasma, and whole blood. The kits evaluated included the Puregene DNA isolation kit (PG), Generation Capture Column kit, MasterPure DNA purification kit, IsoQuick nucleic acid extraction kit, QIAamp blood kit, and NucliSens isolation kit (NS). All six kits evaluated effectively removed PCR inhibitors from each of the four specimen types and produced consistently positive results down to a spiked concentration of 200 PFU of whole CMV per ml. However, the NS and PG resulted in the most consistently positive results at the lowest concentrations of spiked CMV (4 and 0.4 PFU/ml) and, in this evaluation, offered the most sensitive methods for extracting CMV DNA from the four different spiked specimens. Processing time and cost were also evaluated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.