ArticleJournal of clinical microbiology2000
Comparison between plasma and whole blood specimens for detection of Aspergillus DNA by PCR.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Review
- Molecular Diagnostic Testing for Aspergillus.Journal of clinical microbiology · 2016Review
- Comparison of Performance Characteristics of Aspergillus PCR in Testing a Range of Blood-Based Samples in Accordance with International Methodological Recommendations.Journal of clinical microbiology · 2016Article
- Diagnostic Modalities for Invasive Mould Infections among Hematopoietic Stem Cell Transplant and Solid Organ Recipients: Performance Characteristics and Practical Roles in the Clinic.Journal of fungi (Basel, Switzerland) · 2015Review
- Analytical Comparison of In Vitro-Spiked Human Serum and Plasma for PCR-Based Detection of Aspergillus fumigatus DNA: a Study by the European Aspergillus PCR Initiative.Journal of clinical microbiology · 2015Article
- Molecular and nonmolecular diagnostic methods for invasive fungal infections.Clinical microbiology reviews · 2014Review
- Establishment and application of real-time quantitative PCR for diagnosing invasive aspergillosis via the blood in hematological patients: targeting a specific sequence of Aspergillus 28S-ITS2.BMC infectious diseases · 2013Article
- Multicenter comparison of serum and whole-blood specimens for detection of Aspergillus DNA in high-risk hematological patients.Journal of clinical microbiology · 2013Article
- Laboratory diagnosis of invasive aspergillosis: from diagnosis to prediction of outcome.Scientifica · 2013Review
- Article
- Analysis of performance of a PCR-based assay to detect DNA of Aspergillus fumigatus in whole blood and serum: a comparative study with clinical samples.Journal of clinical microbiology · 2011Article
- Clinical utility and development of biomarkers in invasive aspergillosis.Transactions of the American Clinical and Climatological Association · 2011Article
- Surgery of pulmonary aspergillomas in immunocompromised patients.Thoracic surgical science · 2010Article
- Comparison of whole blood, serum, and plasma for early detection of candidemia by multiplex-tandem PCR.Journal of clinical microbiology · 2010Article
- Clinical and laboratory signs as dengue markers during an outbreak in Rio de Janeiro.Infection · 2008Article
- Assessment of Aspergillus fumigatus burden in pulmonary tissue of guinea pigs by quantitative PCR, galactomannan enzyme immunoassay, and quantitative culture.Antimicrobial agents and chemotherapy · 2008Article
- Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis.BMC ophthalmology · 2008Article
- Nucleotide sequencing for diagnosis of sinusal infection by Schizophyllum commune, an uncommon pathogenic fungus.Journal of clinical microbiology · 2006Article
- Evaluation of molecular diagnostic assays for fungal infections.The Journal of molecular diagnostics : JMD · 2006Article
- In vitro release by Aspergillus fumigatus of galactofuranose antigens, 1,3-beta-D-glucan, and DNA, surrogate markers used for diagnosis of invasive aspergillosis.Journal of clinical microbiology · 2006Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ninety-six plasma and whole blood specimens from nine selected patients were analyzed for the presence of Aspergillus DNA. Nineteen specimens from three patients with proven aspergillosis were PCR positive in both materials, whereas an additional 22 were PCR positive in whole blood only. All 36 samples from six patients without signs of aspergillosis were negative in both assays. We conclude that although plasma and whole blood spiked with Aspergillus conidia showed an identical lower detection limit (10 CFU), the sensitivity of plasma PCR was lower than that of PCR performed on whole blood samples.
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