ArticleJournal of clinical microbiology2000
Brachyspira aalborgi infection diagnosed by culture and 16S ribosomal DNA sequencing using human colonic biopsy specimens.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- Clinical and Pathologic Factors Associated With Colonic Spirochete (Brachyspira pilosicoli and Brachyspira aalborgi) Infection: A Comprehensive Systematic Review and Pooled Analysis.American journal of clinical pathology · 2023Pooled it
- A Cross-Sectional Study of Potential Antimicrobial Resistance and Ecology in Gastrointestinal and Oral Microbial Communities of Young Normoweight Pakistani Individuals.Microorganisms · 2023Article
- Association betweenGut · 2021Article
- Spirochete Flagella and Motility.Biomolecules · 2020Review
- Isolates from Colonic Spirochetosis in Humans Show High Genomic Divergence and Potential Pathogenic Features but Are Not Detected Using Standard Primers for the Human Microbiota.Journal of bacteriology · 2019Article
- The comparison of the intensity of human intestinal spirochetes betweenJournal of clinical biochemistry and nutrition · 2019Article
- Human intestinal spirochetosis mimicking ulcerative colitis.Clinical journal of gastroenterology · 2018Article
- Intestinal spirochaetes (genus Brachyspira) colonise wild birds in the southern Atlantic region and Antarctica.Infection ecology & epidemiology · 2015Article
- Appendiceal spirochaetosis in children.Gut pathogens · 2013Article
- Brachyspira species and gastroenteritis in humans.Journal of clinical microbiology · 2013Article
- Rapid and accurate diagnosis of human intestinal spirochetosis by fluorescence in situ hybridization.Journal of clinical microbiology · 2009Article
- High prevalence of spirochetosis in cholera patients, Bangladesh.Emerging infectious diseases · 2009Article
- Comparison of prevalence and risk factors for faecal carriage of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli in four Australian populations.Epidemiology and infection · 2006Article
- Restriction site tagged (RST) microarrays: a novel technique to study the species composition of complex microbial systems.Nucleic acids research · 2003Article
- [Human intestinal spirochetosis].Der Pathologe · 2003Review
- Detection by PCR and isolation assays of the anaerobic intestinal spirochete Brachyspira aalborgi from the feces of captive nonhuman primates.Journal of clinical microbiology · 2003Article
- NotI passporting to identify species composition of complex microbial systems.Nucleic acids research · 2003Article
- Diagnostic examination of human intestinal spirochetosis by fluorescent in situ hybridization for Brachyspira aalborgi, Brachyspira pilosicoli, and other species of the genus Brachyspira (Serpulina).Journal of clinical microbiology · 2001Article
- Comparative prevalences of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli as etiologic agents of histologically identified intestinal spirochetosis in Australia.Journal of clinical microbiology · 2001Article
- BPP43_05035 is aGut microbesArticle
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we report on the isolation and characterization of the intestinal spirochete Brachyspira aalborgi using human mucosal biopsy specimens taken from the colon of a young adult male with intestinal spirochetosis. A selective medium, containing 400 microg of spectinomycin/ml and 5 microg of polymyxin/ml was used for the isolation procedure. A high degree of similarity, in terms of phenotypic properties and 16S ribosomal DNA sequence, was observed between the isolated strain, named W1, and the type strain, 513A, of B. aalborgi. A similarity of 99.7% in the nucleotide sequence was found between W1 and 513A(T), based on the almost-complete gene. A short segment of the 16S rRNA gene was amplified by PCR using genetic material enriched from paraffin-embedded biopsy specimens, which were taken from the patient on two occasions. The products showed 16S rRNA gene sequences virtually identical to that of strain 513A(T) in the actual region. Immunohistochemistry was performed on the colonic biopsy specimens with a polyclonal antibody raised against an intestinal spirochete isolated in a previous case of human intestinal spirochetosis. The antibody reacted strongly with the spirochete on the luminal epithelium. No immune reaction was seen within or below the surface epithelium. Routine histology did not reveal signs of colitis. Electron microscopy showed spirochetes attached end-on to the colonic mucosal surface. The isolate grew poorly on a commonly used selective medium for intestinal spirochetes, which may explain previous failures to isolate B. aalborgi.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.